This study aimed at evaluating the relationship between biomarkers of oxidative stress (OS) in seminal plasma and sperm motility in bulls before and after cryopreservation. Three ejaculates per bull were collected from 20 young bulls. Each ejaculate was analyzed for motility before and after cryopreservation (by CASA), and the SP concentration of Advanced Oxidation Protein Products (AOPP), thiols, and carbonyl groups (CT) were examined. Then, based on their motility, the ejaculates were grouped into: high motility fresh (HMF), low motility fresh (LMF), high motility thawed (HMT), and low motility thawed (LMT) groups. Higher AOPP and thiol concentrations on SP were related (p < 0.05) to the higher LIN and BCF and lower ALH of fresh semen. In addition, AOPP and thiols were significantly higher in HMF than LMF. As a confirmation of this, the Receiver Operating Characteristic (ROC) curve analysis showed that AOPP and thiol concentrations in SP were able to discriminate between HMF and LMF ejaculates (Area Under the Curve of 71.67% and 72.04%, respectively). These observations give an alternative perspective on the relationship between sperm motility and the OS parameters of SP, which need further investigations.

No abstract

The ideal sperm sorting technique should be rapid, cheap and easy to use. It should allow for isolation of sperm with high velocity, reduce reactive oxygen species (ROS) generation and morphological defects as well as the percentage of sperm with fragmented DNA (Asghar et al., 2014). Because centrifugation will increase the exposure of motile spermatozoa to ROS, this processing step should be avoided (Len et al., 2020). Swim-up and density gradient centrifugation are conventional sperm sorting techniques routinely used in artificial reproduction technologies (ART) because they meet many of these requirements. Both these techniques, however, include a

No abstract