In recent years, interest in examining the chemical composition andpharmacological properties of apple cider vinegar in synergy with the applicationof natural products in the pharmaceutical, food and cosmetics industry has beengrowing. The apple vinegar shows a wide range of biological activities(antimicrobial, antioxidant, anti-diabetic, anti-inflammatory, antihypertensive,immune-stimulatory, anticancer) and it has been used in traditional medicine for along time. It consists of 8 essential amino acids (phenylalanine, isoleucine, leucine,lysine, methionine, threonine, tryptophan and valine), organic acids, enzymes,minerals (potassium, calcium, phosphorus, copper, grape and boron) and vitamins(provitamin C, vitamin A, E, B1, B2, B6, vitamin P and provitamin Beta-carotene).The aim of the study was to examine the antibacterial activity of traditionallyproduced apple cider vinegar and to determine whether it exhibits bactericidal orbacteriostatic activity. The results of the study confirmed the antibacterial activityof apple cider vinegar produced with the traditional method. Its antibacterialactivity is in the range of 11.33mm to 14mm.