Genetic variation in three Croatian donkey populations, Istrian (IS), North Adriatic (NA) and Littoral-Dinaric (LD), was analysed using eight microsatellite loci and by sequence and SSCP analysis of the proximal portion of the mtDNA D-loop region. The analysis of microsatellite loci revealed observed heterozygosities in the range of 0.37 (MPZ002 in LD) to 0.85 (AHT21 in LD) and polymorphic information content values in the range of 0.36 (MPZ002 in NA) to 0.78 (AHT21 in LD). The overall probability of exclusion was 0.991. Two populations (IS and NA) were closely related (Fst=0.0034), whereas genetic distances between IS and LD (Fst=0.021) and NA and LD (Fst=0.027) were higher. Using AMOVA, 97.6% of the total genetic variance was portioned within populations, while 2.7% was portioned between the Littoral-Dinaric population and the Istrian/North Adriatic population group. Sequencing of the proximal part of the mtDNA D-loop region revealed 36 polymorphic sites representing 19 haplotypes which clustered into three haplotype groups (Y, W, Ws). Only the Y haplotype was found in the IS population which is characterized by a large body size. Haplotypes W and Ws were found in the NA and LD populations which include smaller animals. All three haplotypes were found in the LD population, indicating sporadic migration events from the IS into LD donkey population.
This experiment investigated the effect of olive cake in the feed of weaned Pramenka lambs on their daily gain, carcass traits and on the chemical composition of different groups of muscles. For that purpose, 45 male lambs were divided into three groups with 15 lambs each and fed separately for 50 days. The first group (control) was fed a commercial concentrate, while the second and third (test) groups were fed a concentrate with the addition of 15% and 30% olive cake, respectively. The results showed that the high level of olive cake inclusion (30%) decreased (P < 0.01) daily gain, final weight, empty carcass weight and (P < 0.05) dressing percentage of lambs. The olive cake resulted in a significantly higher (P < 0.01) proportion of stomach and intestines in the carcass compared to the control. The content of fat, proteins and dry matter in all the analyzed groups of muscles was lowest in the carcasses of lambs fed olive cake. The results of this research suggest that the addition of 15% of olive cake to the concentrate had no significant negative effect on daily gain, carcass weight and dressing percentage of lambs.
The phylogenetic layout of the genotyped (30 microsatellite) 18 sheep breeds in this study demands and provides the opportunity to evaluate both neutral and adaptive components of genetic diversity in a naturally and artificially selected and subdivided sheep population. Seven Pramenka strains from Bosnia and Herzegovina and Croatia characterized by a very low intensity of artificial selection, preserved the highest neutral genetic variability. Eight central and north-western European breeds under considerable artificial isolation and selection preserved the lowest genetic variability. Only combinations of various phylogenetic parameters offer a reasonable explanation for underlying evolutionary forces working in the investigated island and mainland sheep breeds under variable natural and artificial selection. More than 60% of total genetic, diversity was allocated to virtually unselected Pramenka strains, and an additional 25% to native moderately selected Graue Gehoernte Heidschnucke and intensively selected Ostfriesische Milchschafe. Some economically very important breeds and strains did not contribute to a pool with maximal genetic diversity, while they play an important role in the cultural heritage of respective countries.
-Genetic parameters of number of piglets born alive (NBA) and gestation length (GL) were analyzed for 39798 Hungarian Landrace (HLA, 141397 records) and 70356 Hungarian Large White (HLW, 246961 records) sows. Bivariate repeatability animal models were used, applying a Bayesian statistics. Estimated and heritabilitie repeatabilities (within brackets), were low for NBA, 0.07 (0.14) for HLA and 0.08 (0.17) for HLW, but somewhat higher for GL, 0.18 (0.27) for HLA and 0.26 (0.35) for HLW. Estimated genetic correlations between NBA and GL were low, -0.08 for HLA and -0.05 for HLW.
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