Content of proteins and minerals (Mg, Ca, Cu, Zn, Mn and Co) in leaves, stem and root of stinging nettle collected from different localities from the Republic of Macedonia were determined. Generally, the higher content of proteins and minerals were determined in leaves, followed by the content in stem and root. In the quantity of proteins significant differences were determined depending to the organ of stinging nettle. The highest determined values for the protein content in leaves expressed to the dry mass were 26.89% in leaves, 14.54% in stem and 10.89% in root. The values for the calcium content in stinging nettle were higher in comparison to the magnesium values. Zink content in the leaves was two and fi ve times higher than the content of copper and manganese, respectively. In leaves, stem and root non signifi cant differences in cobalt content were determined.

The objective of this study was to determine the gross composition, proteolysis, and volatile and texture profiles during ripening of industrial (IND) and traditional (TRD) Beaten (Bieno sirenje) cheeses made by using ewe milk. In the course of the analyses, statistical differences were determined in some physicochemical parameters, nitrogen fractions, and total free amino acid levels between TRD and IND types of cheese. Higher levels of proteolysis were observed in IND cheeses than in TRD cheeses during ripening. Levels of residual β- and αs-caseins were 72.2 and 48.7%, respectively, in 180-d-old TRD cheeses. However, the residual levels were 52.8% for β-casein and 18% for αs-casein in IND cheeses. Similar differences were noted for the reversed-phase HPLC peptide profiles of 2 types of cheeses. Also, higher concentrations of peptides were eluted in IND cheeses than in TRD cheeses during ripening. A total of 73 volatile compounds, including alcohols (16), esters (17), acids (14), terpenes (7), ketones (5), aldehydes (4), and miscellaneous (10) were identified. The IND cheeses contained higher levels of carboxylic acids, esters, alcohols, and terpenes than the TRD cheeses; however, the same levels of methyl ketones were determined in the 2 types of cheeses at the end of ripening. These may be due to some differences (e.g., pasteurization and scalding temperature, among other factors) in the manufacture of the 2 types of Beaten cheeses. The textural profile of Beaten cheeses showed that TRD production method resulted in firmer, less fracturable, and stiffer cheeses than the IND production method. In conclusion, the results suggest that the use of industrial production method (pasteurization of cheese milk and curd scalding at 70°C) in the manufacture of Beaten ewe milk cheese enriched the volatile profile of the cheese.

Chemical composition of seeds of C. maxima D. and C. pepo L. cultivated in the Republic of Macedonia and physico-chemical characteristics, fatty acid profiles, and sterol and tocopherol contents in pumpkin seed extracts were determined. Higher kernel yield and content of moisture, ash, total nitrogen, proteins and carbohydrates were measured in the C. pepo than in C. maxima seed. The highest extract yield of 487.4 g/kg dry matter was obtained from C. pepo kernel, while 388.2 g/kg dry matter was extracted from C. maxima kernel, when n-hexane was used as solvent. In all extracts, the palmitic, stearic, oleic and linoleic acids were predominant. The linoleic/oleic acid ratio was higher in C. maxima extracts. D7-Sterols were predominant in all extracts, while D5-sterols content was higher in the whole seed than in the kernel extracts. Higher tocopherol content was determined in the extracts of C. pepo whole seed and kernel (153.79 mg/kg and 117.81 mg/kg, respectively), than in those of C. maxima (121.24 mg/kg and 117.55 mg/kg, respectively). In all extracts γ-tocopherol content was higher than α-tocopherol.

Obtained levels of reduction of all tested microorganisms present good base for the production of safe osmotically dehydrated products of animal origin.

Phytochemical research based on ethnopharmacology is gaining interest in industries such as functional food, nutraceuticals, cosmetics and pharmaceutical industries. Plants and plant extracts are a rich source of bioactive secondary metabolites. These compounds are often involved in plant protection against biotic or abiotic stresses. The exploitation of available technologies should be oriented and intensified to extend and enhance the continued usefulness of the plants as renewable sources of chemicals, especially medicinal compounds. This current contribution is focused on extraction and analytical techniques for their isolation from the oregano species, their characterization and their potential antioxidative, as well as their antimicrobial, antifungal and anticarcinogenic properties. The work is structured rendering to the different steps involved in the research; starting with extraction and sample preparation, followed by discussing the analytical techniques employed for the isolation and identification of compound/s responsible for the biological activity and methods and techniques for biological activity assessment.