The phenolic fraction and other polar compounds of the Hibiscus sabdariffa were separated and identified by HPLC with diode array detection coupled to electrospray TOF and IT tandem MS (DAD-HPLC-ESI-TOF-MS and IT-MS). The H. sabdariffa aqueous extract was filtered and directly injected into the LC system. The analysis of the compounds was carried out by RP HPLC coupled to DAD and TOF-MS in order to obtain molecular formula and exact mass. Posterior analyses with IT-MS were performed and the fragmentation pattern and confirmation of the structures were achieved. The H. sabdariffa samples were successfully analyzed in positive and negative ionization modes with two optimized linear gradients. In positive mode, the two most representative anthocyanins and other compounds were identified whereas the phenolic fraction, hydroxycitric acid and its lactone were identified using the negative ionization mode.

Introduction Novel marine compounds supposed an unexplored source of new drugs and expand the possibilities of finding active molecules in all the different signatures of cancer, such as proliferation, invasion and metastases. Here we report the capacity of four marine invertebrate extracts that inhibit proliferation, invasion and migration in colon cancer cell model. Material and methods HCT116 cell line was cultured and exposed to four marine invertebrate extracts (CR from a red coral, PS from an holothurian and NA and NB from nudibranchs) at different concentrations for 24 hour and IC50 was calculated by MTT assay to estimate cytotoxic activity. The rate of cell proliferation and migration was monitored in realtime with the xCELLigence system with E and CIM-16 plates, respectively (Roche Diagnostics GmbH, Germany), which were set in a humidified incubator and maintained in 5% CO2 at 37°C. The impedance value of each well was automatically monitored by the xCELLigence system for 72 hour and expressed as a cell index (CI) value. The anti-invasive rate was determined by Corning BioCoat Matrigel Invasion Chambers with 8.0 mm PET Membrane 24-well Plates (Corning, NY, USA), and crystal violet assay was used to calculate CI. Results and discussions The four marine invertebrate extracts exhibited pronounced cytotoxic and antiproliferative effect in HCT116 model at different doses. The migration assays revealed that CR and especially NB extracts reduced the migration ability of HCT116 cells.Invasion assay let us to isolate superinvasive populations derived from HCT116 which lead to cell models bearing different invasive capabilities (parental (P); invasive subpopulation cells (invaded through membrane four times (I4); and superinvasive line 9 (I9)). Invasive subpopulations I4 and I9 presented a higher rate of invasion than parental population. NB extract showed the best antimigratory capacity compared to other extracts and therefore was selected for further invasive assays. NB showed to reduce invasive cell index in all superinvasive populations. Conclusion The results support the antiproliferative, antimigration and anti-invasive activity of CR, PS, NA and NB marine invertebrate extracts in the highly invasive colon cancer model HCT116. Due to its strong activity, further studies will be focused on the potential of NB extract in the inhibition of metastases-related processes. Introduction Marine invertebrate organisms have received great attention from the scientific community due to its richness in new bioactive compounds with potential anti-proliferative and anticancer activities. In the present study, the cytotoxic efficacy of four marine invertebrate-extracts against human adenocarcinoma colon cancer cells was investigated. Material and methods HT29, SW480 and HGUE-C-1 human colon adenocarcinoma cell lines were cultured and exposed to 4 marine invertebrate extracts at different concentrations for 24 hour. The anti-proliferative properties were examined by MTT assay. Analysis of apoptosis and cell cycle stages wer...