This paper investigates determinants of the survival of new businesses and, in particular, focuses on the impacts of urbanisation, education, and gender of the entrepreneurs. The database used holds information related to new companies created in Andalusia (Spain) in the 2009–2014 period using the incubation services offered by the regional administration. From the methodological point of view, econometric survival methods for duration data are used in the analysis. The article contributes towards the literature on business survival by showing that gender and education play a moderating role in the impact of urbanisation economies on business survival.