No abstract

Vegetables are rich sources of vitamins, minerals and fibres. They have beneficial anti-oxidative effects. The consumption of vegetables containing heavy metals is a significant cause of many diseases. In this study, the concentrations of heavy metals in the frequently consumed vegetables sold in Gwagwalada central market , Abuja, Nigeria was investigated. Quantitative analysis of heavy metals was done using Atomic Absorption Spectrophotometer (AAS). The concentration level of lead showed that cucumber, cabbage, banana, lettuce, green peas, carrot, and onion had 8.65 ± 1.1, 0.0, 100.40 ± 10.0, 68.00 ± 7.0, 64.76 ± 5.8, 61.28 ± 5.6, and 8.92± 1.1, respectively. There was no traceable level of nickel in the fruits and vegetables while cadmium was observed to be 0.10 ± 0.1 in cabbage alone. The study showed that the fruits and vegetables sampled were contaminated with heavy metals higher than the WHO permissible limits.

Icacina trichantha tuber, traditionally acclaimed for its efficacy in the treatment of food poisoning, constipation, diabetes mellitus, malaria, and impotence was in this study evaluated for hypoglycaemic activity in alloxan‐induced diabetic rats, and also the hypoglycaemic principle was isolated and characterized. In vitro toxicity studies was conducted on MDA‐MB‐435 human melanoma, HT‐29 human colon cancer and 3T3‐L1 mouse embryonic fibroblast cell lines while in vivo acute and chronic toxicity studies were performed with varying doses of the extract in rats. The effects of I. trichantha tuber extract on blood glucose levels were investigated in vivo using the oral glucose loading and pancreatic β‐cells destruction with alloxan monohydrate. Thirty (30) rats weighing between 150 and 200 g were randomly assigned to 5 groups (A–E) of 6 rats each. Group A (negative control) was given distilled water (10 ml/kg), group B (positive control) received glibenclamide (2 mg/kg) while groups C, D and E were administered 200, 400 and 600 mg/kg of the extract respectively. The active fractions were isolated following further bioassay‐guided chromatographic separation techniques. Data collected were analyzed using one‐way analysis of variance.. Mean differences were considered significant at p < 0.05. The extract was tolerated by the rats up to the dose of 5000 mg/kg per os. Icacina trichantha tuber extract caused a significant (p < 0.05) dose‐dependent reduction of blood glucose at 400 mg/kg and 600 mg/kg (per os) when compared to the control group at 6 hours, 12 hours and on days 7, 14 and 21of treatment. The tuber extract also improved the symptoms of Diabetes mellitus by significantly (p < 0.05) increasing the serum HDL and reducing serum LDL and triglycerides. The 400 mg/kg extract and 600 mg/kg extract resulted to a significant (p < 0.05) decrease in serum triglycerides levels on 21st day when compared to the diabetic control group. Chronic administration of I. trichantha tuber extract in feed did not show any significant (p < 0.05) difference in the haematological parameters nor was there any marked histopathological lesion. Five compounds were isolated from Icacina trichantha tuber extract viz 17‐Hydroxy‐icacinol, Icacinol, Humirianthol, Humirianthenolide C, and Icacenone. 17‐Hydroxy‐icacinol exerted about 48% reduction of hyperglycemia in diabetic rats and thus identified as the most active. All five compounds displayed significant cytotoxicity on the MDA‐MB‐435 cell line (IC50 0.66–6.44 μM), while Icacinol, Humirianthol, and Humirianthenolide C exhibited cytotoxic activity on the HT‐29 cells (IC50 3.00 – 4.94 μM). This study validates the folkloric use of the tuber and led to the isolation of three 9βH‐pimarane diterpenes (17‐Hydroxy‐icacinol, Icacinol, and Humirianthol), and two 17‐nor‐9βH‐pimarane diterpenes (Humirianthenolide C, and Icacenone) from the tuber of I. tricantha.Support or Funding InformationInstitute of International Education of the United States, Fulbright Junior Scholar Development Award (Grantee ID No. 15120356).This abstract is from the Experimental Biology 2019 Meeting. There is no full text article associated with this abstract published in The FASEB Journal.

The effects of Pausinystalia yohimbe extract and xylazine on the onset of sleep, duration of sleep and vital parameters were used as investigating parameters. Twenty (20) red Sokoto bucks were divided into four (4) groups consisting of five (5) bucks per group. Control group 1 (20mg Xylazine); Group 2 (10, 20, 30 mg plant extract); Group 3 (Plant extract and xylazine); Group 4. Different doses of Pausinystalia yohimbe extract were administered at 5 minutes pre-treatment time of xylazine HCI (20mg/kg). There was significant (p < 0.05) increase of different doses of Pausinystalia yohimbe in respiratory rate, pulse rate and temperature but a significant (p<0.05) decrease on combination of xylazine and Pausinystalia yohimbe extract.