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BackgroundWalking is an integral part of Scotland's National Physical Activity Strategy, and the charity Paths for All's Workplace Step Count Challenge is a flagship programme within this strategy to promote physical activity. Effectively promoting physical activity requires collaborative engagement between stakeholders. However, there is limited guidance on how to do this. The aim of this case study is to share an example of a partnership between Paths for All and researchers to inform the development and delivery of the Workplace Step Count Challenge.MethodAn overview of the partnership, example activities, reflections on opportunities and challenges, and suggestions for future partnership working are considered.ResultsThe partnership has evolved and strengthened over time through building trust. Many of the research activities provide an evidence base for the intervention. This work is mutually beneficial providing support for the work of the organisation, and opportunities for researchers to undertake “real world” research, leading to formal outputs and funding. The “real world” nature is challenging to integrate the most robust research designs. Recommendations for developing future partnerships were identified.ConclusionPromoting physical activity effectively requires partnership working, and this paper provides insight into how such partnerships can work to inform future collaborations.