Mnyusiwalla et al., 2007). Furthermore, nanotechnology has gained a great deal of attention from the researchers in current times because of its multitasking in various fields.In recent years increased chemical usage in management of insect pests and plant pathogens has impacted several environmental issues include environmental pollution (Dhaliwal et al., 2010), pest resistance, pest resurgence and residual phytotoxicity problems etc. (Dhaliwal et al., 2010). To reduce the environmental pollution impact caused by chemical pesticides or fungicide

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Groundnut stem rot caused by Sclerotium rolfsii is a major constraint as it affects the productivity. Although managing this disease using synthetic fungicides is a more feasible method, environmental pollution and side effects caused by them demand some safe fungicides. Seven phytochemicals piperine, quercetin, reserpine, atropine sulfate, β‐sitosterol, ethyl protocatechuate and salicylic acid were initially tested against S. rolfsii under in vitro methods. All the compounds exhibited significant effects on mycelial inhibition (except atropine sulfate), sclerotial development, ooze formation, maturity, sclerotial number and germination of S. rolfsii. The more active compounds, piperine, reserpine and β‐sitosterol were then evaluated under glasshouse condition by adopting various application methods (seed treatment, foliar application and micro‐injection at 2000 μg/mL) on groundnut plants challenged with and without S. rolfsii. All the treatments effectively reduced the plant mortality when tested every 15 days of infection with S. rolfsii. However, the magnitude of reduction varied among the treatments, with the mortality ranging between 9.37 % and 29.68 % compared to the control (40.68 %). The piperine‐microinjected plants recorded minimum mortality (3.12 %). The defense enzymes (PAL and PPO) and key end products such as phenolics (total and individual) were determined in the leaf samples collected after 24, 48 and 72 h of infection with S. rolfsii to understand the systemic resistance induction effect. An increase in PAL and PPO activity was observed in all the samples. While microinjection of β‐sitosterol caused a maximum PAL induction, piperine caused a maximum PPO activity. Further, samples of piperine treated group showed higher induction of phenolic acids (86.46 μg g−1 micro‐injection) compared to β‐sitosterol and reserpine groups in all the methods. When the samples were analysed (HPLC) for individual phenolic acids, maximum accumulation of various acids was observed in the samples collected after 48 h. Tannic and gallic acids were found to be accumulated in higher quantities in most of the samples. The maximum accumulation of phenolic acids was found in micro‐injected samples. These results verified the reduction of mortality through the induction of defensive chemicals by the action of phytochemicals. Thus, the study recommends the use of these natural molecules for the integrated management of stem rot of groundnut after necessary field trials.

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