Aim:The aim was to study the seroprevalence and efficacy of the different serological tests used for detection of antibody against Brucella species in small ruminants of Banaskantha district of North-Gujarat.Materials and Methods:Total 1000 serum samples comprising of 485 from sheep and 515 from goat tested for detection of antibodies against the Brucella species by three different serological tests viz., Rose bengal plate test (RBPT), Standard tube agglutination test (STAT), and Indirect Enzyme-linked immunosorbent assay (I-ELISA).Results:The seroprevalence of brucellosis in small ruminants was 11.30%, 11.10%, and 8.80% by RBPT, STAT, and I-ELISA, respectively. The seroprevalence of brucellosis was found to be higher in sheep than goats. The sensitivity of RBPT was found slight more than STAT, but the specificity of both tests was same. In this study, the overall agreement of RBPT and STAT with I-ELISA was found 92.50% and 92.30% in small ruminants, respectively.Conclusion:I-ELISA was a better serological test as compared to RBPT and STAT in the sense of sensitivity, specificity, and rapidity and it could be advocated for screening of brucellosis in sheep and goats.

The study was carried out on a total of 250 Gir cattle, which covered those presented to the Veterinary Clinical Complex of the College in Junagadh (Gujarat) for therapeutic measures and some from Gaushala near to Junagadh region. The animals were screened for common hemoprotozoan infection based on blood smear examination to record the prevalence rate of infection. The overall prevalence of hemoprotozoan infections recorded on the basis of microscopic examination of blood smears was 35.20 %. Out of these, 64 (25.60%) were positive for Theileria spp., 20 (8%) for Babesia spp., and 4 (1.6%) for Anaplasma spp. a highest prevalence was recorded in April (64.70%), followed by March (57.14%) and February (42.85%). The lowest prevalence was recorded in the month of December (5.88%). The highest prevalence was recorded in the summer season (40.71%), followed by rainy (34.37%) and winter season (19.56%). The highest prevalence of hemoprotozoan infection (41.86%) was recorded in Gir cattle of 3 to 8 years age group followed by 32.35% in 6 months to 3 years age group, and the lowest prevalence was recorded (24.32%) in older animals 8 years and above age group.

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The present study was undertaken to know the hematological alteration in Theileria infected Gir and Jaffrabadi calves in and around Junagadh, Gujarat. Young calves (less than one year of age) with suspected clinical signs of hemoprotozoan infection, presented at the Veterinary Clinical Complex of the College in Junagadh, were taken in this study. Out of 64 suspected cases examined, 13 (20.31%) were found positive for Theileria infection by microscopic blood smear examination. The prevalence of Theileria infection was more in the rainy season (27.27%) followed by summer (26.31%) and least in the winter season (8.69%). Theileria infection was significantly (p less than 0.05) higher in young calves of less than 6 months of age (31.0 %) as compared to calves of 6–12 months of age (9.1%). A nonsignificant higher infection was recorded in Gir calves (22.22%) than Jaffrabadi calves (17.85%). There was no effect of breed, sex or season on Theileria infection in Gir and Jaffrabadi calves in and around Junagadh. Hematological studies revealed anemic condition and increased total leukocyte count with neutrophilia and lymphocytopenia.