Background: Multiple myeloma (MM) is a malignant neoplasm of clonal plasma cells. Neutrophil-to-lymphocyte ratio (NLR) and platelet-to-lymphocyte ratio (PLR) are prognostic factors and biomarkers of systemic inflammation. The aim of this study was to determine a correlation between NLR, PLR, and hematological parameters in patients with MM. Methods: The clinical data of 50 MM patients were collected from hospital medical records. NLR and PLR were calculated from data obtained from clinical records. Results: The median age was 60 years at diagnosis. The study cohort was divided into two groups based on cutoff points taken from previously published data (NLR: 2.56 and PLR: 157). Erythrocyte sedimentation rate, blood urea, uric acid, platelet count, and absolute neutrophil count were higher in patients with high NLR than in patients with low NLR. A significant association between NLR and blood urea was seen (P < 0.0018). Pearson's correlation analysis revealed a strong positive correlation for blood urea and NLR, uric acid and NLR, and blood urea and PLR. Conclusion: Thus, combined applications of NLR and PLR could be used as a cost-effective diagnostic predictor of MM patients.

Background: Multiple myeloma (MM) is a clonal plasma cell disorder characterized by heterogeneous complex genetic abnormalities. Due to the low proliferative index of plasma cells, conventional cytogenetic (CC) analysis is hampered in MM. Interphase fluorescence in situ hybridization (FISH) along with CC enhances the sensitivity of detection. The study aims to investigate the diagnostic yield and prevalence of P53 deletion in patients with MM. Materials and Methods: Cytogenetic analysis and FISH were performed on 41 MM patients. Results: Our study showed that 55–65 years of age range among all individuals, predominantly affected by the disease. The cytogenetic analysis detected abnormal karyotype in 12% (5/41), normal karyotype in 66% (27/41), and culture failure in 22% (9/41). Abnormal karyotype showed numerical abnormalities such as hyperdiploidy 5% (n = 2) and hypodiploidy 7% (n = 3%). Chromosomes 5, 9, 11, and 21 were common gains among hyperdiploid cases. Chromosome 7, 17, 22 and Y were the common missing chromosome in hypodiploid cases. P53 gene deletion is a rare genetic event and difficult to identify using CC. FISH analysis of deletion 17p was detected in 15% (6/41). Out of six cases, two cases showed deletion of 17p region, three cases showed monosomy 17, and one case showed amplification signals for chromosome 17. Conclusion: CC along with FISH increases the rate of detection of abnormality in MM cases. P53 being less frequent is uncommon at initial diagnosis; increasing its incidence with advanced stage is considered one of the important prognostic factors in MM.