No abstract

The route of migration and development of Ancylostoma ceylanicum, when given orally, has been described for the first time in hamsters. A predominant feature of the migration is the absence of any lung journey. This is unlike A. caninum, A. duodenale and A, brazilicnse when larvae are similarly given to an abnormal host. The larvae of A. ceylanicum apparently had no submucosal migration

Summary Background High‐mobility group box 1 ( HMGB 1) is a damage‐associated molecular‐pattern protein. Stevens–Johnson syndrome ( SJS )/toxic epidermal necrolysis ( TEN ) are serious, immune‐mediated skin‐blistering conditions. Objectives To determine serum and/or blister‐fluid total HMGB 1 levels in SJS / TEN cohorts, and HMGB 1 expression in formalin‐fixed, paraffin‐embedded ( FFPE ) SJS / TEN skin vs. healthy and maculopapular exanthema ( MPE ) skin. Methods Serum HMGB 1 was quantified in Malawian nevirapine‐induced hypersensitivity, Taiwanese SJS / TEN and Spanish SJS / TEN cohorts. FFPE skin (healthy skin, MPE , SJS / TEN ) was stained and assessed for HMGB 1 expression. Results Serum total HMGB 1 was not significantly elevated in patients with nevirapine‐induced SJS / TEN (3·98 ± 2·17 ng mL −1 ), MPE (3·92 ± 2·75 ng mL −1 ) or drug reaction with eosinophilia and systemic symptoms (4·73 ± 3·00 ng mL −1 ) vs. tolerant controls (2·97 ± 3·00 ng mL −1 ). HMGB 1 was significantly elevated in Taiwanese patients with SJS / TEN , highest during the acute phase (32·6 ± 26·6 ng mL −1 ) vs. the maximal (19·7 ± 23·2 ng mL −1 ; P = 0·007) and recovery (24·6 ± 25·3 ng mL −1 ; P = 0·027) phases. In blister fluid from Spanish patients with SJS / TEN , HMGB 1 (486·8 ± 687·9 ng mL −1 ) was significantly higher than in serum (8·8 ± 7·6 ng mL −1 ; P <0·001). Preblistered SJS / TEN skin showed decreased epidermal nuclear HMGB 1 expression in upper epidermis vs. healthy or MPE skin but retained basal/suprabasal expression. ...

Sustained demand for dermatologic care throughout military medicine, in conjunction with increasing dermatologic provider shortages, has led to increase use of teledermatology in military treatment facilities (MTFs). Initially used to aid in the differentiation of suspicious melanocytic lesions, dermoscopy has found increasing clinical utility in an expanding realm of general dermatologic conditions. We demonstrate the use of synchronous teledermoscopy within a remote MTF by repurposing webcam technology already available at most MTFs. Two patients were seen in clinic at a remote naval primary care clinic with limited subspecialties. Once written consent was retrieved, an on-site dermatologist evaluated each patient and performed a history and skin exam with dermoscopy. Synchronous consultations were conducted with the Global Med Cart (GlobalMed(R) Clinical Access Station with TotalExam(R) 3 HDUSB camera), and Cisco webcam video jabber (Cisco TelePresence PrecisionHD USB Camera part number TTC8-03). The patients then underwent individual synchronous teledermatology consultations with an off-site U.S. Navy dermatologist located in the continental United States. The methodology for the consultation involved the use of a standard dermatoscope and jabber webcam. Two synchronous teledermatology consultations were completed successfully on patients in MTFs with limited subspecialty capabilities. Both cases, with two lesions of concern per case, had 100% concordance between the on-site and teleconsulted dermatologist. Through observing inter-rater agreements between the on-site and remote dermatologists, this small study demonstrates a novel application of technology readily available at most MTFs.

Chitosan is a deacetylated product of chitin produced by chitin deacetylase, an enzyme that hydrolyses acetamido groups of N-acetylglucosamine in chitin. Chitosan is a natural polymer that has great potential in biotechnology and in the biomedical and pharmaceutical industries. Commercially, it is produced from chitin via a harsh thermochemical process that shares most of the disadvantages of a multistep chemical procedure. It is environmentally unsafe and not easily controlled, leading to a broad and heterogeneous range of products. An alternative or complementary procedure exploiting the enzymatic deacetylation of chitin could potentially be employed, especially when a controlled and well-defined process is required. In this study, 20 strains of bacteria were isolated from soil samples collected from different beaches of Chennai, India. Of these 20 bacterial strains, only 2 strains (S3, S14) are potent degrader of chitin and they are also a good producer of the enzyme chitin deacetylase so as to release chitosan.