Chemical composition of essential oils (EOs) of Calycolpus genus have been reported in the literature. However, there is only 1 report about volatile profile from Calycolpus goetheanus. This work aims to evaluate the seasonal and circadian influences on EO composition and yield of C. goetheanus collected in Marajó Island, Brazilian Amazon. For the circadian study, the leaves were collected in January (rainy season) and July (dry season) every 3 hours during a period of 15 hours. The EOs were obtained by hydrodistillation and their chemical compositions analyzed by gas chromatography coupled to mass spectrometry and flame ionization detectors. The major compound identified in all EO samples was 1,8-cineole with amounts ranged from 14.4% (January, 6 am) to 33.0% (July, 3 pm). The highest average of 1,8-cineole was obtained during circadian study of the dry season (25.5% ± 5.8%) and the lowest during the circadian study of the rainy season (15.6% ± 1.5%). The multivariate analysis grouped the samples into 2 different groups: Group I characterized by the lowest amounts of 1,8-cineole (16.0%-18.7%), and Group II characterized by a higher content of 1,8-cineole (24.0%-33.0%). The oil yield and chemical composition did not show relationship with climate parameters (solar radiation, humidity, and temperature). Therefore, there was only quantitative variability in the EOs compositions during the circadian rhythm evaluated on dry and rainy seasons.

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