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Superparamagnetic iron oxide nanoparticles (SPIONs) ranging between 20-40 nm were synthesized by co-precipitation method. Functionalized SPIONs were coated with FITC-labeledstreptavidin. Biotinylated aflatoxin B1 specific anti-aflatoxin B1 antibodies were prepared, separately. The fluorophore FITC-labeled-streptavidin-SPIONs and biotinylated anti-aflatoxin B1 antibodies were allowed to interact to obtain immuno-SPIONs bioconjugates. The extract of Aspergillus flavus and Aspergillus parasiticus infected groundnut seeds was tested to detect the aflatoxin B1 molecules (<5 ng/mL) by forming immuno-SPIONs complex. The immuno-SPIONs bioconjugates were characterized and evaluated by gel electrophoresis, Fourier transform infrared spectroscopy, Fluorescence microscopy and Transmission electron microscope using appropriate controls. The bioconjugated FITC-labeled-STV-SPION particles were found to be stable over 2 months at 4 C.

The emerging concept of thread-based microfluidics has shown great promise for application to inexpensive disease detection and environmental monitoring. This paper describe the properties of thread for the development of thread based low-cost microfluidics device for the diagnostic, food safety and environmental assay. Various properties of thread like capillary flow, wicking capacity and absorption capacity, make them important to fabricate the low-cost microfluidics device. In this paper, three different thread sample viz. cotton yarn, cotton thread and spun thread were taken and different properties like wicking capacity, fluid capillary flow, absorption capacity were measured as well as Scanning Electron Microscopic (SEM) analysis of the same was also carried out to study the morphology and texture of thread fibers. Data obtained from result indicate that the cotton thread having all the ideal characteristic to fabricate the diagnostic device and thus it is suitable for the fabrication of thread based microfluidics device for the diagnostic purpose.

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