Millettia peguensis is commonly known as Moulmein rosewood, is a legume tree species in the genus Millettia belongs to the family Fabaceae. The powdered leaf was successively extracted with Petroleum ether through Soxhlet apparatus. The leaves of Petroleum ether extract of Millettia peguensis by GC-MS analysis clearly showed the presence of 10 compounds. In GC-MS analysis, Millettia peguensis showed 3 major and 7 minor compounds. The results revealed that Pentadecane (32.73%), Tetradecane (29.79%) and Octadecane(22.77%) were reported as 3 major components in the Petroleum ether leaves extract of the Millettia peguensis. The seven minor compounds such as Eicosane (7.23%), Undecane, 5-methyl-(2.13%), 9-methylheptadecane (1.40%), Sulfurous acid, dodecyl hexyl ester (1.13%), Heptadecane, 2,6,10,15-tetramethyl-(0.99%), 2-Bromo dodecane (0.96%) and Heneicosane (0.87%) were also reported from leaves. The results indicated that the leaf extracts might be utilized as natural drug for the treatment of several infectious diseases.