Pumpkin seed (Cucurbita pepo L.) is a nutritionally valuable food and a significant source of income globally. Pumpkin seeds are rich in oil, protein, unsaturated fatty acids and tocopherols, which are associated with improved human health. Understanding the genetic diversity among pumpkin accessions varying in seed nutrition traits is necessary for designing sound breeding strategies for developing superior cultivars. In the current study, 26 simple sequence repeats (SSR) markers were used to assess genetic relatedness among 29 C. pepo accessions varying in seed oil, seed protein, seed-coat phenotype, seed size and fatty acid composition. The SSR markers revealed 102 alleles averaging 3.92 alleles per loci and mean polymorphic information content (PIC) of 0.44. Eleven of the markers had a PIC of ≥0.5. Ward dendrogram and principle component analysis based on seed traits grouped the genotypes into two major clusters corresponding to subspecies pepo and texana, with all the reduced-hull accessions grouping within the former. Collectively, this data suggests wide phenotypic (seed traits) and genotypic variation within C. pepo that may be exploited to develop superior reduced-hull cultivars.

Vegetable growers are keen on cost-cutting measures to increase profitability. Containerized vegetable production can be done in a shade-house or garden, and it often requires commercial potting media. Although expensive, potting media are lightweight and provide high water- and nutrient-holding capacities, and thus they are widely used by growers. Growers often discard or compost the potting media after a single season due to issues such as diseases, pests, and weeds. However, old potting media could be reused for containerized production if appropriately sterilized and amended with fertilizer salts. The current study was conducted to determine the feasibility of using sterilized recycled potting medium amended with fertilizer salts for containerized production of squash. This new 4-page publication of the UF/IFAS Horticultural Sciences Department was written by Marie Dorval, Riphine Mainviel, Vincent Michael, Yuqing Fu, Bala Rathinasabapathi, and Geoffrey Meru.https://edis.ifas.ufl.edu/hs1404