Purpose The purpose of this research is to study the clinical outcomes using a next-generation sequencing-based protocol allowing for simultaneous testing of mutations in the beta thalassemia (HBB) gene, including single nucleotide polymorphism (SNP) markers for PGT-M along with low-pass whole genome analysis of chromosome aneuploidies for PGT-A. Methods A combined PGT-M (thalassemia) plus PGT-A system was developed for patients undergoing IVF in Vietnam. Here we developed a system for testing numerous thalassemia mutations plus SNP-based testing for backup mutation analysis and contamination control using next-generation sequencing (NGS). Low -pass next-generation sequencing was used to assess aneuploidy in some of the clinical PGT cases. Patients underwent IVF followed by embryo biopsy at the blastocyst stage for combined PGT-A/M. Results Two cases have completed the entire process including transfer of embryos, while a further nine cases have completed the IVF and PGT-M/A analysis but have not completed embryo transfer. In the two cases with embryo transfer, both patients achieved pregnancy with an unaffected, euploid embryo confirmed through prenatal diagnosis. In the further nine cases, 39 embryos were biopsied and all passed QC for amplification. There were 8 unaffected embryos, 31 carrier embryos, and 11 affected embryos. A subset of 24 embryos also had PGT-A analysis with 22 euploid embryos and 2 aneuploid embryos. Conclusions Here we report the development and clinical application of a combined PGT-M for HBB and PGT-A for gross chromosome aneuploidies from 11 patients with detailed laboratory findings along with 2 cases that have completed embryo transfer.

The antioxidant and hypoglycemic capacities of various solvent fractions from the trunk-bark methanol extract of Terminalia alata Heyne ex Roth (T. alata) were investigated. The DPPH radical scavenging assay was used to evaluate the antioxidant activity, and the methods for determination of digestive enzymes inhibitory activity and fasting blood glucose reduction capacity in diabetic rats were used to determine the hypoglycemic activity of the extract fractions. The results indicated that higher total phenolics content was measured with increasing polarity of extraction solvent and DPPH radical scavenging activity coincided with phenolics content. Ethyl acetate fraction (EAF), n-butanol fraction (BF), and water fraction (WF) were obtained. They possessed α-amylase inhibition with the IC50 values of 0.056 ± 0.001, 0.138 ± 0.005, and 0.022 ± 0.001 mg.mL-1, respectively, which were lower than acarbose (IC50 = 0.154 ± 0.02 mg.mL-1). In contrast, IC50 values of α-glucosidase inhibitory activities of these fractions were higher than those of acarbose. In addition, these fractions also lowered fasting blood glucose concentrations in streptozotocin induced diabetic rats at a dose of 200 mg.kg-1 bw (body weight) without inducing body weight loss, which was not observed when treating with acarbose. The EAF and WF of trunk-bark of T. alata are recommended as potent sources for further research on antioxidant and hypoglycemic activities.

Bài viết tập trung làm rõ thực trạng thể chất nam sinh viên 18 tuổi tại tỉnh Sóc Trăng; thông qua việc tổng hợp và so sánh giá trị trung bình các tiêu chí đánh giá thể chất nam sinh viên 18 tuổi tại tỉnh Sóc Trăng và trung bình thể chất Việt Nam (TBTCVN) 18 tuổi; từ đó, đánh giá thể lực nam sinh viên năm nhất các trường cao đẳng tại tỉnh Sóc Trăng theo quyết định 53/2008/QĐ-BGD&ĐT của Bộ Giáo dục và Đào tạo.

Bài viết lựa chọn được 5 test, qua đó ứng dụng đánh giá trạng thái tâm lý trước thi đấu của nam vận động viên 15-17 tuổi đội tuyển vovinam Thành phố Hồ Chí Minh. Kết quả nghiên cứu có 4 vận động viên có trạng thái tâm lý sẳn sàng thi đấu; 11 vận động viên sốt xuất phát và 5 vận động viên ở trạng thái thờ ơ chưa sẳn sàng thi đấu.

No abstract