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Genome annotation is the process of estimation of biological features from a Genomic Data. The target of a genome annotation is to identify the key features of the genome sequence particularly, the genes and gene products. The characteristics of gene, its products, gene prediction programs of Aspergillus niger are discussed. Although the number of genomes in Genomic databases are increasing day by day, genome wide analyses is afflictive depending on the quality of the genome annotations. This study illustrates the importance of integrative approaches for automatic annotations of genomes of Aspergillus niger by computational method. However, the annotation process is more complicated in Eukaryotes; we used a comparative study for Gene prediction by FgenesH algorithm by various software providers. The final annotation of Aspergillus niger CBS 513.88. has been created as a GB file in Artemis, A Sequence viewer and annotation tool developed in Sanger Institute.

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