It is an evergreen, spiny shrub or small tree 5 to 6 m in height. The plant has single or multiple stems and irregular branches covered with smoothish brown to gray bark. The twigs are quadrangular (when young), green, and bare sharp axillary spines 3 to 17 mm long. The leaves are yellow-green to dark green, with 5-to 28-mm winged petioles and elliptic to oval leathery 4-to 13-cm long blades with edges that have minute rounded teeth. The crushed foliage has a strong,

Juglans regia linn belongs to family Juglandaceae. It is commonly known as Walnut tree. Juglans regia bark has been claimed to possess anti-inflammatory, blood purifying, anticancer, depurative, diuretic and laxative activities. The bark is finely powdered and used to prevent bleeding gums and as a mouth rinse. The present investigation deals with microscopic evaluation of bark and establishment of its quality parameters, including physicochemical, phytochemical evaluation, HPTLC analysis & Microbial load. Chief microscopic characters include cork, phloem fibres with stone cells & calcium oxalate crystals. Phytochemical screening revealed presence of reducing sugars; alkaloids; tannins & phenols; steroids & saponins. The bark powder was found to be free from pathogenic organisms. The study will provide referential information for the correct identification of the crude drugs.