Tooth extraction is dental procedure that causes wound in oral cavity. Kirinyuh leaves (Chromolaena odorata) contain flavonoids, saponins and tannins which help the wound healing after tooth extraction. Purpose of study was to determine effect of giving kirinyuh leaf ethanol extract on wound healing after tooth extraction in guinea pigs (Cavia cobaya) in angiogenesis. Sixty male guinea pigs were divided into five groups: aquadest, iodine glycerine (positive control), kirinyuh 2.5%, 5%, and 10%. Each group consisted of 12 guinea pigs in each group. Kirinyuh leaf extract was prepared in a topical gel with CMC-Na as the solvent. Left mandibular incisor of guinea pigs was extracted then treated according to the group every day. Guinea pigs were euthanized on days 3, 7, 10, and 14 (three guinea pigs from each group per day) and made histological sections with hematoxylin eosin staining. Angiogenesis was observed with binocular microscope equipped with camera. Results showed that number of new blood vessels in kirinyuh 10% group reached highest number faster (day 7) than the other groups (p=0.000). This study concluded that kirinyuh leaves accelerate angiogenesis by increasing the number of blood vessels. The 10% concentration is the most effective kirinyuh extract concentration to accelerate angiogenesis.