RESUMO -Avaliou-se a eficiência da lignina purificada e enriquecida (Lipe ® ) como indicador externo para estimar a digestibilidade dos nutrientes de dietas em eqüinos em comparação aos métodos de coleta total de fezes e do indicador óxido crômico. Foram utilizadas seis potras Mangalarga Marchador, com média de 2 anos de idade e 345 kg de peso vivo, alimentadas com feno de alfafa e concentrado comercial na proporção de 50:50, para ingestão de matéria seca de 3,1%PV, e sal mineral.O experimento teve duração de 29 dias, de modo que os 24 dias iniciais foram destinados à adaptação dos animais à dieta e às instalações e os cinco finais para coleta total das fezes. Utilizou-se delineamento em blocos casualizados, no qual cada animal constituiu um bloco e cada método de determinação da digestibilidade, um tratamento. Os cálculos de produção fecal e digestibilidade de cada nutriente foram feitos utilizando-se os indicadores e a taxa de recuperação fecal de cada nutriente.Os coeficientes de digestibilidade dos nutrientes obtidos utilizando-se o óxido crômico foram superiores aos da coleta total e Lipe ® . Os resultados obtidos com Lipe ® foram similares aos determinados por coleta total. O método do óxido crômico é inadequado, enquanto o Lipe ® é eficiente para estimar a digestibilidade de nutrientes da dieta em eqüinos.Palavras-chave: cavalo, consumo, dieta, indicadores, matéria seca Validation of Lipe ® as method to evaluate the apparent digestibility of nutrients in equines ABSTRACT -The objective of this work was to test the efficiency of enriched and purified lignin, Lipe ® , as external indicator in order to estimate the apparent digestibility of the nutrients in equine diets, compared with the total feces collection and the chromic oxide methods. Six female Mangalarga Marchador with average of two years of age and average weight of 331 kg were used. The diet was alfalfa hay, commercial grain and mineral salt. The experimental period was of 29 days, the first 24 used for the adaptation of animals to the diet and facilities and the last five days for feed estimation and total feces collection. A random blocks design was used in which each animal constituted one block and each digestibility determination method, one treatment. The digestibility of nutrients using chromic oxide were smaller than those from the total collection and Lipe ® , and those estimated by the Lipe ® were similar to those from the total collection. Chromic oxide revealed to be inadequate and Lipe ® was considered efficient to estimate the apparent digestibility in equine.Key Words: diet, dry matter, horse, intake, markers IntroduçãoNa nutrição animal, o conhecimento do valor nutritivo dos alimentos é importante para formulação de dietas balanceadas. Para avaliar os nutrientes que devem ser aproveitados pelo animal, pode-se determinar a digestibilidade aparente (porção do nutriente consumido passível de ser digerida no trato gastro intestinal). De acordo com Meyer 1995), a individualidade do animal, a composição química da dieta, o nível de cons...
Introduction: Canine visceral leishmaniasis (CVL) is an endemic disease in Brazil, and integrated control actions have been adopted by the Brazilian Ministry of Health to control its spread. However, the transmission profile is unknown in areas with recent CVL cases, including Itaúna, located in the Brazilian state of Minas Gerais, where the present study was carried out. Methods: A total of 2,302 dogs from 12 neighborhoods were serologically tested for canine VL using the current diagnostic protocol adopted by the Brazilian Ministry of Health. Test positivity rate (TPR) and CVL prevalence were determined for each neighborhood. The presence of Leishmania was assessed in 60 seropositive dogs which had been recommended for euthanasia. Twenty-two of them (37%) were asymptomatic, and 38 (63%) were symptomatic for CVL. Parasitological (myeloculture and smear/imprint) and molecular (PCR) methods were employed for Leishmania detection in bone marrow, spleen, mesenteric lymph nodes, and ear skin. The infecting Leishmania species was identified by DNA sequencing. Results: CVL prevalence (per 1,000 dogs) varied from 0.0-166.67, depending on the neighborhood, with a mean of 68.96 (SD 51.38). Leishmania DNA was detected in at least one tissue from all seropositive dogs, with comparable TPR among tissues. Leishmania parasites were identified in most (54/60) seropositive dogs, and the infecting parasite was identified as Leishmania infantum in all of these. Conclusions: Prevalence of CVL is a contributor to the spread of visceral leishmaniasis in Itaúna.
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