The mechanism by which stem cell-based therapy improves heart function is still unknown, but paracrine mechanisms seem to be involved. Adipose-derived stem cells (ADSCs) secrete several factors, including insulin-like growth factor-1 (IGF-1), which may contribute to myocardial regeneration. Our aim was to investigate whether the overexpression of IGF-1 in ADSCs (IGF-1-ADSCs) improves treatment of chronically infarcted rat hearts. ADSCs were transduced with a lentiviral vector to induce IGF-1 overexpression. IGF-1-ADSCs transcribe100-to 200-fold more IGF-1 mRNA levels compared to nontransduced ADSCs. IGF-1 transduction did not alter ADSC immunophenotypic characteristics even under hypoxic conditions. However, IGF-1-ADSCs proliferate at higher rates and release greater amounts of growth factors such as IGF-1, vascular endothelial growth factor (VEGF), and hepatocyte growth factor (HGF) under normoxic and hypoxic conditions. Importantly, IGF-1 secreted by IGF-1-ADSCs is functional given that Akt-1 phosphorylation was remarkably induced in neonatal cardiomyocytes cocultured with IGF-1-ADSCs, and this increase was prevented with phosphatidylinositol 3-kinase (PI3K) inhibitor treatment. Next, we tested IGF-1-ADSCs in a rat myocardial infarction (MI) model. MI was performed by coronary ligation, and 4 weeks after MI, animals received intramyocardial injections of either ADSCs (n = 7), IGF-1-ADSCs (n = 7), or vehicle (n = 7) into the infarcted border zone. Left ventricular function was evaluated by echocardiography before and after 6 weeks of treatment, and left ventricular hemodynamics were assessed 7 weeks after cell injection. Notably, IGF-1-ADSCs improved left ventricular ejection fraction and cardiac contractility index, but did not reduce scar size when compared to the ADSC-treated group. In summary, transplantation of ADSCs transduced with IGF-1 is a superior therapeutic approach to treat MI compared to nontransduced ADSCs, suggesting that gene and cell therapy may bring additional benefits to the treatment of MI.

Recent studies have identified adipose tissue as a new source of mesenchymal stem cells for therapy. The purpose of this study was to investigate the therapy with adipose-derived stromal cells (ASCs) in a rat model of healed myocardial infarction (MI). ASCs from inguinal subcutaneous adipose tissue of male Wistar rats were isolated by enzymatic digestion and filtration. Cells were then cultured until passage 3. Four weeks after ligation of the left coronary artery of female rats, a suspension of either 100 µl with phosphate-buffered saline (PBS) + Matrigel + 2 ´ 10 6 ASCs labeled with Hoechst (n = 11) or 100 µl of PBS + Matrigel (n = 10) was injected along the borders of the ventricular wall scar tissue. A sham-operated group (n = 5) was submitted to the same surgical procedure except permanent ligation of left coronary artery. Cardiac performance was assessed by electro-and echocardiogram. Echo was performed prior to injections (baseline, BL) and 6 weeks after injections (follow-up, FU), and values after treatment were normalized by values obtained before treatment. Hemodynamic measurements were performed 6 weeks after injections. All infarcted animals exhibited cardiac function impairment. Ejection fraction (EF), shortening fractional area (SFA), and left ventricular akinesia (LVA) were similar between infarcted groups before treatment. Six weeks after therapy, ASC group showed significant improvement in all three ECHO indices in comparison to vehicle group. In anesthetized animals dp/dt + was also significantly higher in ASCs when compared to vehicle. In agreement with functional improvement, scar area was diminished in the ASC group. We conclude that ASCs improve cardiac function in infarcted rats when administered directly to the myocardium.

Analisa os principais mecanismos mitocondriais envolvido no processo de envelhecimento humano. Por meio de revisão narrativa de literatura, cujos descritores foram “mitocôndrias”, “envelhecimento humano” e “radicais livres”, nas bases de dados PubMed, Web of Science, Scielo e Google Acadêmico, entre 2009 à 2019. Após aplicar os critérios de exclusão, foram selecionados 19 artigos. . Concluiu-se que a hipótese mais aceita é a da Teoria dos Radicais Livres, o declínio da biogênese mitocondrial e a disfunção mitocondrial afetam a integridade e funcionalidade celular, resultando no envelhecimento do indivíduo.

Objetivo: Avaliar as evidências disponíveis na literatura sobre a atuação do Enfermeiro e da equipe multidisciplinar no controle da hipertensão através da educação em saúde. Métodos: Este estudo adotou a revisão integrativa da literatura como forma de pesquisa utilizando as bases de dados da Biblioteca Virtual de Saúde (BVS) e Google Acadêmico com a combinação dos descritores HAS, Enfermeiro, equipe multidisciplinar, educação em saúde. Resultados: Foram revisados sete estudos que abordavam o tema e a análise destes apontaram a importância da educação em saúde no controle da hipertensão arterial por meio do profissional Enfermeiro, assim como a abordagem da equipe multiprofissional e o apoio da família na adesão ao tratamento. Considerações finais: Infere-se que o profissional Enfermeiro e a equipe multidisciplinar, somado ao comprometimento do usuário, são os principais responsáveis pelo tratamento do paciente portador de HAS, através da educação em saúde, estimulando a prática do autocuidado dando adesão ao tratamento, e aproximando os portadores de HAS aos serviços de saúde. A continuidade dos estudos neste tema é necessária para o desenvolvimento de planejamentos estratégicos de ações básicas de saúde aos portadores de HAS.

Os detritos marinhos representam uma ameaça crescente aos ambientes costeiros e oceânicos. Nos últimos anos este resíduo tem ganhado destaque devido aos graves danos causados ao ambiente. O presente estudo realizou uma análise quantitativa e qualitativa dos resíduos sólidos encontrados na região costeira de Barra de Caravelas, BA. O material coletado foi resultado de campanhas realizadas em conjunto com as ONGs Patrulha Ecológica – Escola da Vida e Instituto Baleia Jubarte. O plástico foi o constituinte mais abundante, totalizando 2.972 kg, seguido de tecido (709,4 kg), metal (352,3kg), isopor (246,7 kg), vidro (246 kg) e borracha (217,8 kg). Os outros resíduos não foram tão significativos quando comparados aos citados, exceto o nylon (131 kg). Nota-se a importância de avaliar a poluição por lixo na região costeira de Caravelas, BA, uma vez que esses dados podem contribuir para a conservação da biodiversidade.