There is a need for more detailed elucidation of T-cell immunity in chikungunya infection. CD8 T cells are one of main actors against viruses. Here, we analysed CD8 T lymphocytes from patients in the acute and chronic phases of chikungunya disease (CHIKD). Our results demonstrate that CD8 T cells expressed higher ex vivo granzyme B, perforin and CD107A expression in patients in the acute phase of CHIKD compared with healthy individuals and higher ex vivo expression of CD69, interleukin-17A, interleukin-10 and CD95 ligand, and co-expression of CD95/CD95 ligand. These results elucidate the importance of these lymphocytes, demonstrating immune mechanisms mediated in human chikungunya infection.

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Introduction: The use of plants for medicinal purposes is one of the most used resources for the treatment of various diseases. There is a lack of awareness on the part of consumers that such products are frequent causes of hospital admissions resulting in public health problems. Thus, aiming at the importance of phytochemical and biological analyzes of

Carvacrol, a phenolic monoterpene, has diverse biological activities, highlighting its antioxidant and antihypertensive capacity. However, there is little evidence demonstrating its influence on vascular regeneration. Therefore, we evaluated the modulation of carvacrol on endothelial repair induced by endothelial progenitor cells (EPC) in hypertension. Twelve-week-old spontaneously hypertensive rats (SHR) were treated with a vehicle, carvacrol (50 or 100 mg/kg/day), or resveratrol (10 mg/kg/day) orally for four weeks. Wistar Kyoto (WKY) rats were used as the normotensive controls. Their systolic blood pressure (SBP) was measured weekly through the tail cuff. The EPCs were isolated from the bone marrow and peripherical circulation and were quantified by flow cytometry. The functionality of the EPC was evaluated after cultivation through the quantification of colony-forming units (CFU), evaluation of eNOS, intracellular detection of reactive oxygen species (ROS), and evaluation of senescence. The superior mesenteric artery was isolated to evaluate the quantification of ROS, CD34, and CD31. Treatment with carvacrol induced EPC migration, increased CFU formation and eNOS expression and activity, and reduced ROS and senescence. In addition, carvacrol reduced vascular ROS and increased CD31 and CD34 expression. This study showed that treatment with carvacrol improved the functionality of EPC, contributing to the reduction of endothelial dysfunction.

<p>As zonas costeiras são espaços muito afetados pela alta concentração demográfica, apresentando, por isso, diversos problemas ambientais. As praias, constituintes desses espaços, acabam, por sua vez, perdendo muitas de suas funções, tanto em termos de importância para o homem, principalmente em relação ao lazer, como em termos de importância ambiental, como parte integrante dos ecossistemas. Nesse contexto, os esgotos domésticos se apresentam como um dos principais degradadores do ambiente praiano, causando danos ao meio ambiente, à economia e à saúde da população, afetando a balneabilidade, por exemplo. Nesse sentido, o presente trabalho objetivou fazer uma investigação sobre o comprometimento ambiental da balneabilidade da praia de Manaíra em João Pessoa-PB, desenvolvendo um estudo da frequência da impropriedade da praia para o banho e avaliando parâmetros físico-químicos e microbiológicos de caracterização das águas e de efluentes advindos de galerias de águas pluviais que deságuam naquele ambiente. Com o trabalho pode-se concluir que a alta frequência com que a praia permanece imprópria ao banho está relacionada a ligações clandestinas de esgotos domésticos à rede de águas pluviais. Também foi possível concluir que esse lançamento indevido pode estar trazendo grande contaminação por microorganismos patogênicos e amônia (NH3-N).</p>