The study, was carried out during March – June 2022 at Department of Horticulture, Naini Agricultural Institute, SHUATS, Prayagraj. The experiment was carried out to see the performance of the most suitable soil media for growth and establishment of Papaya seedlings. There were nine treatments including control viz, M0 Control (Garden soil), M1(Soil + FYM), M2 (Soil+FYM+biocapsules), M3 (Soil+FYM+Neemcake), M4  (Soil + FYM + Neemcake + biocapsules), M5 (Soil +FYM + Vermicompost), M6 (Soil + Sand + Vermicompost + biocapsules), M7 (Soil + FYM + Sand) M8 (Soil+FYM+Sand+Biocapsules) replicated three times with 10 seeds per replication. Growth medium significantly affected the growth, biomass, and root morphological indexes of papaya seedlings. The results reveal that the treatment M6 (Soil+Sand+Vermicompost+biocapsules) was the most suitable treatment over all the other treatments in relation to germination and growth parameters.