The Brazilian flora is one of the richest in biodiversity and has many species with high pharmaceutical and nutraceutical potential. Among these species is ora-pro-nobis (Pereskia aculeata Mill.), which stands out for the high protein content found in its leaves. The exogenous application of amino acids in crop production has shown promising results, such as the increase in productivity and plant quality, besides their capacity in alleviating environmental stress in plants. Exogenous application of L-arginine (0, 0.25, 1.0 and 2.0 g L−1 of water) was performed by drenching on coconut fiber growing media of ora-pro-nobis plants. Plant growth parameters analyzed were chlorophylls and carotenoids contents, crude protein content, and determination of enzymes and oxidative stress substances. The higher concentration of L-arginine (2 g L−1 of water) increased net photosynthetic rate, leaf area, plant fresh and dry mass, carotenoids content and crude protein content in leaves. Thus, the exogenous application of L-arginine in ora-pro-nobis plants can improve both the productivity and nutritional value of its leaves.
Heat stress can impact crop development and yield and amino acids play diverse essential roles in plants. This work aimed to study the long-term effects of foliar spray with L-arginine in antioxidant machinery, physiology, nutrition, productivity and fruit quality of tomato plants subjected to transient heat stresses. Six concentrations of L-arginine were sprayed on the plants: 0 (control), 0.10, 0.25, 0.50, 1.0 and 2.0 g•L -1 . The content of hydrogen peroxide (H 2 O 2 ), a reactive oxygen species, decreased concurrently to the increasing arginine concentration. The ascorbate peroxide (APX) activity had an inverse behavior to that observed for H 2 O 2 content (r = -0.79), not only indicating that arginine is able to modulate APX, but also suggesting that this enzyme plays an important role on the mitigation of H 2 O 2 generation under heat stress. Ascorbate peroxide and catalase (CAT) activities had a positive correlation (r = 0.82), showing that these enzymes may work in tandem. The influence of arginine on photosynthesis activity and gas exchange was generally weak and depended mainly on the plant developmental stage. Yield was increased by 19.8 and 23.1% in plants that received 1.0 and 0.5 g•L -1 of arginine, respectively, when compared to control plants. In conclusion, the use of exogenous L-arginine can protect tomato plants against oxidative imbalance under transient heat within protected environments.
Uso de fertilizante de liberação controlada no cultivo de tomate em ambiente protegido / Vivyan Justi Conceição. --versão revisada de acordo com a Resolução CoPGr 6018 de 2011. --Piracicaba, 2022. 42 p. Tese (Doutorado) --USP / Escola Superior de Agricultura "Luiz de Queiroz". 1. Fertilizante de eficiência aprimorada 2. Fertirrigação 3. Solanum lycopersicum L. I. Título DEDICATÓRIA Ao meu filho Vitor, dedico. AGRADECIMENTOSAgradeço ao CNPQ pela bolsa concedida.À empresa Haifa pelo financiamento do meu projeto de doutorado.Ao programa de Pós-graduação em Fitotecnia da ESALQ/USP. Aos meus mentores que me guiaram para que eu chegasse até a conclusão dessa etapa tão importante para mim.À minha família, primeiros professores dos aprendizados que necessitei.Ao meu filho, todo meu amor! Ao Leandro Fidanza, minha gratidão! À Escola Superior de Agricultura Luiz de Queiroz, minha gratidão, respeito e admiração e uma casa para sempre.Á minha orientadora Simone Costa Mello, pela amizade, pelo apoio, pela parceria e pelos ensinamentos.A todos que, durante essa jornada, cheia de desafios, aprendizados e ensinamentos acreditaram em mim e que por algum momento ou situação compartilharam comigo, meu respeito e gratidão.Às portas que se abriram, e também as que se fecharam e às oportunidades que obtive, obrigada por tanto aprendizado! Aos colegas de pós-graduação e aos da graduação que me auxiliaram em algum momento, seja nas atividades de campo ou de alguma outra maneira: muito obrigada.Obrigada Bruna Godinho, estudante de graduação, que me auxiliou como estagiária bolsista na execução do projeto.Ao grupo GEPOL, obrigada pelo apoio. Á Deus, acima de todas as coisas.
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