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Extended cultivation with multiple removal of BC pellicles is proposed herein as a new biosynthetic process for bacterial cellulose (BC). This method enhances the BC surface area by 5–11 times per unit volume of the growth medium, improving the economic efficiency of biosynthesis. The resultant BC gel-films were thin, transparent, and congruent. The degree of polymerization (DP) and elastic modulus (EM) depended on the number of BC pellicle removals, vessel shape, and volume. The quality of BC from removals II–III to VII was better than from removal I. The process scale-up of 1:40 by volume increased DP by 1.5 times and EM by 5 times. A fact was established that the symbiotic Medusomyces gisevii Sa-12 was adaptable to exhausted growth medium: the medium was able to biosynthesize BC for 60 days, while glucose ran low at 24 days. On extended cultivation, DP and EM were found to decline by 39–64% and 57–65%, respectively. The BC gel-films obtained upon removals I–VI were successfully trialed in experimental tension-free hernioplasty.

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A new method for the treatment of chronic pancreatitis by introducing into arterial bed of pancreas or a regional arterial bed pancreas suspension of hydrocortisone. Objective: To provide local intensification anti-inflammatory effects, reducing the risk of systemic side effects. Material and methods: Endovascular treatment of 30 patients (19 males (63,3%) and 11 women (36.7%), the average age of 50.7 years. Organ (gastroduodenal artery) or regional (celiac trunk) infusion of a suspension of hydrocortisone. Indications e ineffectiveness of conservative therapy, recurrent of pain.Clinical evaluation of the dynamics of abdominal pain during the infusion, within 1 month and 6 months. A score of pain, where 0-his absence, the maximum intensity of 5.Studied indices of tissue blood flow in the stomach by a method rheoarteriogastrography at baseline, at the middle and at the end of long arterial infusion suspension of hydrocortisone in pool celiac trunk, n = 15.We studied the performance of the specific density of the pancreatic tissue during the treatment. Results: Severe pain at the time of admission to the surgical hospital. After endovascular treatment of pain intensity reduced to 0,34 AE 0,19 points.Flow condition in the stomach without ischemic changes during and after treatment.According to the CT study noted a decrease in the size of the pancreas, the restoration of the specific density of the parenchyma to normal values. Conclusions: Endovascular treatment method allows HP to stop inflammation, affect tissue fibrosis and the loss of its basic functions.

A new method for the treatment of chronic pancreatitis by introducing into arterial bed of pancreas or a regional arterial bed pancreas suspension of hydrocortisone. Objective: To provide local intensification anti-inflammatory effects, reducing the risk of systemic side effects. Material and methods: Endovascular treatment of 30 patients (19 males (63,3%) and 11 women (36.7%), the average age of 50.7 years. Organ (gastroduodenal artery) or regional (celiac trunk) infusion of a suspension of hydrocortisone. Indications e ineffectiveness of conservative therapy, recurrent of pain.Clinical evaluation of the dynamics of abdominal pain during the infusion, within 1 month and 6 months. A score of pain, where 0-his absence, the maximum intensity of 5.Studied indices of tissue blood flow in the stomach by a method rheoarteriogastrography at baseline, at the middle and at the end of long arterial infusion suspension of hydrocortisone in pool celiac trunk, n = 15.We studied the performance of the specific density of the pancreatic tissue during the treatment. Results: Severe pain at the time of admission to the surgical hospital. After endovascular treatment of pain intensity reduced to 0,34 AE 0,19 points.Flow condition in the stomach without ischemic changes during and after treatment.According to the CT study noted a decrease in the size of the pancreas, the restoration of the specific density of the parenchyma to normal values. Conclusions: Endovascular treatment method allows HP to stop inflammation, affect tissue fibrosis and the loss of its basic functions.