The search for new ways to optimize the adaptive capabilities of service dogs is a prerequisite for increasing the viability of animals and increasing their longevity. Dihydroquercetin neutralizes the increased level of free radicals toxic to the body, protecting cell membranes from destruction, and reduces the harmful effects of oxidative stress. The use of dihydroquercetin contributed to increased providing cells with oxygen, stimulated anabolic processes and increased the antioxidant defense of the body. The amount of red blood cells in the blood of service dogs increased by 11.5-19.1% (p<0.01) when using dihydroquercetin, hemoglobin increased by 5.7-9.4% (p<0.05), total protein by 21-32% (p<0.01), albumin by 30-31% (p<0.001), compared with similar data at the beginning of the experiment. The antioxidant properties of dihydroquercetin contributed to the reduction of malondialdehyde by 23.9% (p <0.05); reduction of diene conjugates by 26%; and an increase in catalase activity by 36% compared to the data on the first day of the experiment. The use of dihydroquercetin optimizes the adaptive capabilities of service dogs and can be used to increase the vitality and longevity of animals.
When feeding 30-180-day old calves with mineral sorbent vodnite, the number of red blood cells increases by 4.17% (p≤0.05), the hemoglobin level increases by 9.4% (p≤0.05), total protein contentby 6.44% (p≤0.01), albumin contentby 4.60% (p≤0.001), the phagocytic activity of leukocytesby 12.42% (p≤0.05), the bactericidal activityby 6.47% (p≤0.05), the lysozyme activity -by 10% (p≤0.05), the average daily weight gain of calvesby 7.4%, the live weightby 7.6% (p≤0.01). In fresh cows fed with vodnite, physical and biochemical characteristic improve and the gross milk yield increases by 45-84 kg (p<0.05). Vodnite used for cattle increases the genetic productivity.
In laboratory conditions, the modified nutrient differential diagnostic medium Drigalski agar with lactose is recommended to be used for cultivation (isolation) and differentiation of enterobacteria of the Enterobacteriaceae family. The differentiation of enterobacteria on a modified medium is carried out according to their ability to ferment lactose, mannitol, glucose, sucrose, gelatin and form hydrogen sulfide. The environment can also be used for conducting sanitary and microbiological studies of environmental objects. The environment can be used to perform the ONPG test. In the course of preparing the modified Drigalski agar for operation in laboratory conditions, we recommend using the medium according to one of the options: when one of the carbohydrates is added, the medium is poured into single-section reusable or disposable petri dishes; when two carbohydrates are added, the medium is poured into two-section reusable or disposable petri dishes; when a complex of carbohydrates lactose + mannitol and glucose + sucrose is added, the medium is poured into two-section reusable or disposable petri dishes; when using all four drives separately, the medium is dispensed into single-compartment reusable or disposable petri dishes. We recommend preparing modified Drigalski agar at a plant for the production of ready-made culture media with filling the medium in two-section petri dishes (complex of carbohydrates lactose + mannitol in one section, glucose + sucrose in another section), or with filling the medium in four-section petri dishes (with each carbohydrate in separate section).
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