Objective: The results of the determination of the phenolic compounds in Ruta graveolens L. and Stellaria media (L.) Vill. herb by the method of high-performance liquid chromatography (HPLC) with ultraviolet (UV)-detection are presented in the article.Method: The qualitative composition and quantitative content of the phenolic compounds were studied by HPLC on the Agilent 1200 chromatograph using methanolic extracts of R. graveolens L. and S. media (L.) Vill. herb. The components of hydroxycinnamic acids were determined at wavelengths of 320 nm and 330 nm; flavonoids at 255 nm and 340 nm; and tannins at 255 nm and 280 nm.Results: On the basis of the analysis conducted by the method of HPLC in the herb of R. graveolens L., 16 substances were identified: 6 flavonoids (apigenin, rutin, quercetin, luteolin, isoquercetin, and hyperoside), 4 hydroxycinnamic acids (chlorogenic, rosmarinic, caffeic, and p-coumaric), and 6 tannins (gallic and ellagic acids, gallocatechin, epigallocatechin, epicatechin, and epicatechin gallate). In the herb of S. media (L.) Vill., chlorogenic acid, flavone aglycones: Luteolin and apigenin, and flavonol glycosides: Isoquercetin and rutin were identified.Conclusion: According to the results of the research, it was established that the dominant components of R. graveolens L. and S. media (L.) Vill. herb are chlorogenic acid and a flavonoid component apigenin, which has anti-inflammatory activity. Thus, the obtained results point to the prospect of further research with the aim of creating of herbal substances with a certain pharmacological action.

Objectives: The investigation of antimicrobial activity of water-alcohol plant extracts Ruta graveolens L., Nepeta cataria L., and Stellaria media (L.) Vill. and the detection of the synergism of their antimicrobial action with erythromycin against the skin isolates of Staphylococcus aureus with different mechanisms of acrolides, lincosamides, and streptogramins (MLS)-resistance. Materials and Methods: It has been carried out by the micromethod of diffusion into agar and the method of diffusion of active substances into agar using paper disks. Results: The direct antimicrobial effect of the investigated extracts to the test strains was established. The synergistic antimicrobial effect of 1/4 and 1/64 minimum decreasing concentration of erythromycin to all strains of staphylococcus was demonstrated by extracts from R. graveolens L. The biologically active substances of medicinal plants more effectively restore the sensitivity to erythromycin for staphylococcus with low MLS-resistance through blocking reflex mechanisms. High-level MLS-resistance undergoes to a modification influenced considerably less. Conclusion: The investigated plant extracts of R. graveolens L. show antimicrobial activity against all tested strains of microorganisms: Gram-positive and Gram-negative bacteria, Candida tropicalis. N. cataria L. extract influenced some of the tested cultures of microorganisms. Extract of S. media (L.) Vill. inhibited the growth of microorganisms, mostly to streams of Streptococcus aureus ATCC 25923 and S. aureus ATCC 6538 in a dose of 200 mcg/ml.