Axillary bud explants of Oxalis corniculta grown in the field were excised and inoculated on MS medium with various concentrations and combinations of auxins (2, 4-D and NAA) and cytokinins (BAP and Kn) for indirect shoot formation through callus induction. Indirect organogenesis was achieved by the application of PGRs such as BAP, Kn, and NAA in the culture medium. The maximum mean shoot number (9.2) and regeneration frequency (77%) was found with BAP (2.0 mg/l) and NAA (0.5 mg/l). Healthy microshoots were separated and transferred to rooting medium supplemented with NAA, IBA and IAA. MS medium augmented with NAA 2.0 mg/l was found to be best for rooting. The regenerated plantlets were then hardened, acclimatized and where exhibited 75% survivability. The reproducible protocol can be used for regeneration and genetic transformation studies. Plant Tissue Cult. & Biotech. 32(2): 181-191, 2022 (December)

The multiple shoots (14.2) from Oxalis corniculata differentiated through bud breaking on MS medium with 2.0 mg/l BAP. The regenerated shoots were further sub-cultured on MS medium containing 1.0 mg/l BAP and 0.5 mg/l NAA. Vegetative to reproductive phase transition occurred in the cultures affected by plant growth regulators (PGRs), sucrose concentrations, strength of MS salts and light. Flower buds initiated from the regenerated shoots on half strength of MS salts with 1.0 mg/l Kn + 0.5 mg/l NAA and 3% sucrose. These flower buds opened under low light (10-15 μmol m-2 s-1) condition with 18h/6h photoperiod within 3 weeks of culture initiation. The shoots did not flower in the dark. Though smaller in size, the in vitro generated flowers were morphologically comparable to the natural flowers. Fruits were set in the cultures within 5 weeks. Plant Tissue Cult. & Biotech. 32(2): 193-203, 2022 (December)