We developed a cell suspension culture system for zedoary (`ìêÅìã~=òÉÇç~êá~ Roscoe), using 100 g fresh weight inoculum in a batch culture. The maximum cell biomass of 68.46 g/L fresh weight was obtained after 14 days of culture in a 10 L bioreactor with a pitch-blade impeller maintained at an agitation speed of 150 rpm and an aeration rate of 2.5 L/min. The accumulation of sesquiterpenes and polysaccharide in zedoary cells from 2 to 18 days was measured by HPLC and a phenol-sulfuric acid assay, respectively. The total polysaccharide concentration increased between 2 to 10 days of culture and reached a maximum value of 6.55%. HPLC revealed several eluted peaks of sesquiterpenes, which increased in amplitude from days 2 to 10. Furthermore, our results indicated that biotransformation occurred in the cell suspension, transforming certain sesquiterpenes into other types during culture. © KSBB hÉóïçêÇëW=`Éää=ëìëéÉåëáçå, Curcuma=zedoaria, emi`, éçäóë~ÅÅÜ~êáÇÉ, ëÉëèìáíÉêéÉåÉë=

Three iridoids, asperulosidic acid (1), geniposidic acid (2), and galioside (3) and three anthraquinones, 2‐hydroxymethyl‐3‐hydroxyanthraquinone (4), rubiadin‐1‐methyl ether (5), and anthragallol‐2‐methyl ether (6) were isolated from the methanol extract of the Morinda officinalis leaves. Their chemical structures were elucidated by ESI‐MS, 1D‐ and 2D‐NMR spectra and comparison with the data reported in the literature. Galioside (3) was reported from Morinda genus for the first time.