The objective of this study is to determine the influence of dried egg yolk (DEY) powder on freezability of semen buffalo as an alternative of fresh egg yolk (FEY) with or without taurine. Semen was collected from five buffalo bulls with total motility (≥70% mass motility), pooled, extended (1: 10) with different tris extenders containing 20% FEY versus 5, 10, 15 and 20% DEY (1st experiment). Tris-EY extender contained 15% DEY was supplemented with taurine (0, 25, 50, 75 and 100 mM) in 2nd experiment. Results showed that increases in progressive motility at concentration 10 , 15% than 20% in DEY and FEY respectively, after dilution, equilibration, and thawing, Also, acrosome integrity percentage in post-equilibrated and thawed semen was improved (P <0.05) by 5, 10, and 15% DEY as compared with FEY, being the highest with 15% DEY. Adding 25 or 50-Mm taurine improved progressive motility after dilution, and acrosome integrity after equilibration, and membrane integrity percentage after all stages of cryopreservation. the powdered egg yolk at concentration of 10 or 15% may be used, as an alternative of fresh egg yolk in the diluents of buffalo sperm freezing. Addition of taurine at a level of 25 and 50 mM has possible protective effects as an antioxidant to improve semen quality of buffalo semen extended with 15% dried egg yolk.

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This study aimed to determine the utilizing whey resulting from making paneer cheese as a diluent for bovine semen. Semen was collected from 5 Friesian bulls, pooled, divided into three portions, and diluted (1:10) with three extenders; Tris-soybean lecithin (TSL), whey (W0), or whey+5% egg yolk (WEY), then equilibrated (5 o C for 4 h), and frozen (-196 o C). Semen was evaluated for progressive motility (PM), live sperm (LS), acrosome integrity (AI), abnormality (AB) and curled tail (CT) after dilution,equilibration and thawing using Computer Assisted Semen Analyzers (CASA) system. Results revealed that percentages of PM, LS, AI and CT of spermatozoa were higher (P<0.50), and AB percentage was lower (P<0.05) with WEY-extender than in other extenders either post-dilution or post-equilibration. In post-thawing semen evaluated by CASA, WEY-extender improved (P<0.05) percentages of progressive motility, total motility, rapid progress motility, functional sperm, and sperm immotility as compared to other extenders. WEY and W0 extenders increased (P<0.05) Linearity (LIN) compared with TSL extender. Straight line velocity (VSL) and straightness (STR) were higher (P<0.05) with WEY than TSL-extender, while did not differ between WEY and TSL-extender. Percentages of normal morphology form, abnormality in head, neck, and dual deformation, as well as teratozoospermic and sperm deformity indices were lower (P<0.05) in WEY-extender than in other extenders. Conclusion: Sperm freezability in Friesian bull semen can be improved during cryopreservation by using whey+5% egg yolk extender, as a promising extender, in comparing with whey without egg yolk and tris-citrate extenders.