Infection of grapevines by Phaeoacremonium species, thought to cause young vine decline, is currently causing concern to grape growers worldwide. Symptoms of the disease include chlorotic, sparse and stunted leaves, late bud-burst, uneven growth and undersized trunks, which show internal discolourations and frequently exude shiny, black, tarry substances ('black goo'). This study investigated prevalence of the disease within commercial vineyards in selected grape-growing areas. Grape growers, largely from Marlborough but also from Nelson, Canterbury and Gisborne, contributed vines with decline symptoms for further observation and isolation. Characteristic internal staining of vine trunks was found, usually below the graft union, in 84% of the 70 vine samples. Isolations onto selective agar found Phaeoacremonium spp., most commonly P. chlamydosporum, in 54% of vines and in 20 of the 27 vineyards sampled. Incidence may have been higher but 26% of wood specimens also contained other, fast-growing fungi which overgrew the wood tissues, interfering with attempts to isolate the slowgrowing Phaeoacremonium spp. Isolations from roots of the 33 most severely infected vines found 14 to be also infected with Cylindrocarpon destructans, which causes a root rot called 'black foot'. Further studies are needed into young vine decline and into the epidemiology and control of P. chlamydosporum.
Since 1918, Trinidad and Tobago has been a rich source of biological control agents and has shipped numerous natural enemies both regionally and internationally. Successful classical biocontrol programmes using predominantly predators and parasitoids began in earnest in the 1970s, initially for Aeneolamia varia saccharina and then for Diatraea saccharalis control in sugarcane. Several other pests, including citrus blackfly Aleurocanthus woglumi, diamondback moth Plutella xylostella and pink hibiscus mealybug Maconellicoccus hirsutus, were successfully managed using biocontrol. The use of microbial agents, particularly entomopathogenic fungi, has also been assessed. Metarhizium anisopliae has caused high nymphal and adult mortality in A. varia saccharina, while Paecilomyces tenuipes has caused as much as 67% larval mortality in P. xylostella. Over the past 10 years there has been an increase in the number of exotic insect species in Trinidad and Tobago, and classical biocontrol has played and is expected to keep playing an important role in managing these invasive pests.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.