Small crayfish muscle fibres were voltage clamped and synaptic current elicited by superfused GABA solutions was measured. Analysis of the fluctuations of synaptic current and of relaxations of the current after voltage steps yielded analogous results. The current has two components. The first component is characterized by the opening of synaptic channels with a single channel conductance gamma = 9 pS and an average open time tau = 5 ms, measured at 23 degrees C and - 100 mV. tau depends on the membrane potential, tau E = tau 0 x eE/epsilon, and epsilon was about +100 mV in the average. The channel open time agrees with the time constant of decay of the inhibitory postsynaptic current (IPSC) elicited by a nerve stimulus. The current is carried by chloride ions. The second current component is much slower, the average channel open time was tau s = 33 ms at 23 degrees C and -60 mV. The open time tau s of the slow component also was shortened on hyperpolarization. The reversal potential for the current component was more positive than -50 mV. This slow component also seems to be a synaptic one.

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Deviating from the normal situation, some crayfish muscle fibres showed spontaneous inhibitory activity: discharge of large inhibitory postsynaptic currents, IPSCs, alternating with long lasting bursts of current noise. Analysis of the bursts of current noise revealed that they are composed of spontaneous miniature unit currents, sIPSCs. In the burst periods the sIPSCs occurred with an average rate of 3.5--10 k Hz and had an amplitude of about alpha = 90 pA at a driving force delta E = 10 mV. The peak conductance gamma alpha = alpha/delta E of the sIPSCs was gamma alpha = 9.2 nS +/- 0.5 (S.D., n = 5) for membrane potentials between E = --60 mV and E = --80 mV. gamma alpha seemed to decrease when the membrane was hyperpolarized. The time constants of decay, tau of the sIPSCs were identical with tau of the IPSCs. Further, tau and its potential dependence agreed with the mean lifetimes of inhibitory postsynaptic channels operated by gamma-aminobutyric acid (GABA) [Dudel et al. 1977, 1980]. Synchronized opening of about 750 inhibitory synaptic channels generates a sIPSC. Analysis of this anomalous bursting inhibitory activity thus yields the size of the inhibitory quantum of transmission, which could not be obtained from IPSCs.