Diapause development in Culex tarsalis Coquillett has been characterized by reduced blood-feeding to complete termination of ovariole development at or before stage IIa (Bennington et al. 1958; Schaefer and Washino 1970; Schaefer et al. 1971; Bellamy and Corbet 1973; Mitchell 1979). The role that diapausing Culex species have in overwintering arboviruses has been examined (Eldridge 1966, 1968; Eldridge et al. 1972; Reeves 1974; Eldridge and Bailey 1979; Mitchell 1979). Gonotrophic dissociation and its implications for survival of the vector and virus has been reviewed by Washino (1977). Eldridge (1966) indicated gonotrophic dissociation was possible in Culex pipiens L. incubated at low temperature and short daylength and later reported failure of ovarioles of prehibernating C. pipiens to mature following a blood meal (Eldridge and Bailey 1979). Eldridge et al. (1972) reported that Culex restuans Theobald exhibited gonotrophic dissociation in response to conditioning by short daylength and low temperature. Mitchell (1981) has reported blood-feeding and gonotrophic dissociation in a significant portion of diapausing Culex tarsalis from Colorado, when females were kept at 15°C and at short daylength conditions.

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A total of 72 overwintering Anopheles earlei Vargas was collected from southern Manitoba during December 1980 and 1981. These mosquitoes were maintained at 15 °or 20 °C, and a photoperiod of 8L: 16D. Forty-two females were offered a blood meal and 25 of these fed. Extensive fat body development was seen in the blood-fed females collected in 1980; this was not seen in collections from 1981. Gonotrophic dissociation was observed in 24 of the blood-fed females.