Plants are widely used by all sections of the society either as folk medicines or as pharmaceutical preparation of modern medicine. In vitro propagation of plants holds great promise for conservation and enhancement of valuable medicinal plants. Cleome rutidosperma has been used in indian ayurvedic medicine for the treatment of a wide number of health disorders. The present study deals with the influence of different plant growth regulators (PGR) including kinetin (Kin), 6- Benzylaminopurine (BAP) and 2, 4-Dichlorophenoxyacetic acid (2,4-D) on the growth of plant and the identification and comparison of bioactive constituents of wild and in situ propagated C. rutidosperma plant using Gas Chromatography - Mass Spectrometry analysis (GC-MS). Nodal segments used as explants were cultured on Murashige and Skoog's medium (MS) supplied with different concentrations of PGRs. Multiple shoot generation was achieved after 28 days of incubation. The GC-MS analysis showed the presence of ten compounds of micropropagated and seven compounds of wild plants were identified. The result concluded that various concentration of PGR had a significant role in in vitro regeneration of plant and showed that the phytoconstituents of micropropagated plant is comparatively higher than that of wild plant.

In the recent years, tissue culture has emerged as a promising technique to obtain genetically pure elite populations under in vitro conditions. The Cleome viscosa are used in traditional systems of medicine for the treatment of many diseases in human. The present study aims to investigate the role of assorted plant growth regulators (PGRs) on in vitro propagation and comparison of similar and dissimilar compounds of wild plant C. viscosa. Nodal explants of 1.5-2.0 cm were used to induce multiple shoots in Murashige and Skoog (MS) medium supplemented with various concentration of different plant growth regulators (PGRs) such as 6 -Benzylaminopurine (BAP), Kinetin (KIN), Naphthalene-3-acetic acid (NAA) and the bioactive constituent of wild and in vitro propagated C. viscosa plant was compared by analyzing polar and non polar extract of both the plants using Gas Chromatography -Mass Spectrometry (GC-MS) analysis. Multiple shoots were initiated within 28 days of inoculums and the various concentration of PGR had a significant role in the number of shoot formed and the in vitro regeneration of explants. The regenerated plantlets showed no morphological differences from the wild plant but the GC-MS analysis of ethanol extract showed the presence of eight compounds in wild plant and six in micropropagated while chloroform extract showed ten compounds in both plants.