Expired seed has a very low vigour and viability which can inhibit the sprout growth due to difficulty in imbibition. Bean sprout extract contains more auxin, cytokinin and gibberellic acid that are important to increase growth potential of expired seed. Therefore, it is a must to pay attention to the concentration and soaking duration of bean sprout extract when soaking the seeds. The research was conducted in March to April 2020 at Sei Rengas I Subdistrict, Medan Kota, North Sumatera, using randomized complete block design with two treatment factors. The result showed that the concentration of bean sprout extract significantly affected the parameters of the membrane leakage rate which the best treatment is soaking in aquadest. The soaking duration significantly affected the parameters of the membrane leakage rate and growth speed which the best soaking duration is 12 hours. The interaction of two treatments significantly affected the parameters of the membrane leakage rate which the best combination is soaking in aquadest for 12 hours.
Andaliman is a typical plant in Sumatera Utara that hasn’t been widely cultivated because it’s difficult to germinate. The aim of this research was to study the effect of giving an electric field to the metabolism of andaliman (Zanthoxylum accanthopodium DC) seeds which contributes to accelerating germination. Research conducted at the Laboratory of Plant Ecology, Tissue Culture Laboratory and Molecular and Genetic Laboratory of the Faculty of Agriculture, Universitas Sumatera Utara from June to July 2018 was arranged in a complete non-factorial randomized design repeated three times. Seeds are given by an electric field for 18 days with a voltage of 324 volts. The results of this research showed that the electric field treatment and differences in fruit colour not different significantly on seed metabolism in accelerating germination after do analysis of proteins, APX, SOD and electrical conductivity. Descriptively the highest average of APX was showed on without electric field: red colour seeds 2.39 μM/min/mg protein (P1). The highest average of SOD was showed on with electric field: red colour seeds 485.27 unit/mg protein (P4). The highest average of electrical conductivity was showed on without electric field: red colour seeds 6733.33 μS (P1).
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