The paper describes the necessity to only use tests for national salmonella monitoring and reduction programmes that are "harmonized", i.e. that produce at least at herd level the same results. Four in Germany licensed tests were audited in a proficiency test by four independent and neutral laboratories. The test was designed rather to harmonize the tests, if necessary, than to evaluate the single tests. The methods used to provide a high credibility for the proficiency test's outcome are explained. The conclusion of the ring test is that three of the four tests can be used for the salmonella monitoring programme in Germany as long as not single results are compared to each other, but the results of sets of sera are used for the herd categorization for their risk level of introducing Salmonella spec. into the food chain.
Zusammenfassung Tylosin hemmt den Vermehrungsprozeß von Coxiella burneti im embryonierten Hühnerei. Je später nach der Infektion das Antibiotikum bei einmaliger Inokulation verabreicht wird, desto geringer ist die hemmende Wirkung. Bei mehrmaliger Verabreichung ist die vermehrungshemmende Wirkung umso größer, je häufiger Tylosin verabreicht wird. Selbst bei Verschwinden der Embryoletalität nach Gabe von 2 mg/Ei an 4 Tagen sind aber noch infektionsfähige Coxiellen in den Embryonen vorhanden. An strukturellen Veränderungen unter dem Einfluß von Tylosin während der Vermehrung werden vor allem gehäufte Zellwandablösungen bei großen Partikeln, große geblähte Formen, Störungen in der Innenstruktur der Partikel sowie Knospungsprozesse beschrieben. Summary The effect of Tylosin on multiplication and structure on Coxiella burneti in the incubating hen egg Tylosin inhibits the multiplication process of Coxiella burneti in the embryonated egg. The greater the delay after infection before the antibiotic is administered by a single inoculation the smaller is the inhibition. When the antibiotic is given repeatedly its inhibitory effect increases with the frequency of administration. After dosing with 2 mg./per egg for 4 days infective coxiella are still present in the embryo. The structural changes influenced by Tylosin during multiplication include increased detachment of the cell wall, large inflated forms, damage to the inner structure of the particles and budding process. Résumé Action de la tylosine sur la multiplication et la structure de Coxiella burneti dans des oeufs de poules incubés La tylosine inhibe le processus de multiplication de Coxiella burneti dans les oeufs embryonnés de poule. L'action inhibitrice est d'autant plus faible que l'unique injection d'antibiotique est faite plus tartivement après l'infection. L'inhibition est plus forte lors l'applications fréquentes et répétées de tylosine. Avec la disparition de la létalité embryonnaire après l'application de 2 mg/oeuf en 4 jours, des Coxiella infectieuses se trouvent encore dans les embryons. On trouve avant tout comme modifications structurelles dues à l'influence de la tylosine pendant la reproduction, des destructions de la paroi cellulaire par de grosses particules, des formes gonflées, des troubles dans la structure interne de la particule ainsi que des processus de bourgeonnement. Resumen Acción de la tylosina sobre la multiplicación y estructura de Coxiella burneti en el huevo embrionado de gallina La tylosina inhibe el proceso de multiplicación de Coxiella burneti en el huevo embrionado de gallina. Después de la infección, cuanto más tarde se administra el antibiótico en inoculación única, tanto más escasa resulta la acción inhibidora. Con la administración reiterada, la acción inhibidora de la multiplicación es tanto mayor cuanto más frecuentemente se administre la tylosina. Pero incluso al desaparecer la letalidad embrionaria tras la administración de 2 mg/huevo en 4 días aún se hallan presentes coxielas infestantes en los embriones. De entre las m...
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