Ten females of Kaluga sturgeon (Huso dauricus; 73.8 ± 6.49 kg, total length 204.5 ± 12.8 cm) matured for the first time after a full life-cycle in captivity. Absolute gonad biomass was 8.4 ± 1.3 kg per female and relative fecundity 11.2 ± 1.6%, at maturation stage IV. These fish were induced to spawn by injection of hormone LHRH-A2 at a dosage of 10 lg kg )1 . Male Kaluga was injected one time at 5 lg kg )1 and milt was stripped 16 h after injection of LHRH-A2. Water temperature was about 16 ± 1°C during the induction period. Fertile eggs were obtained 36.8 ± 2.6 h after first injection; they were fertilized by semi-dry method. Fertilization rate was 80.7 ± 3.8%. Fertilized eggs were incubated McDonald jars (hatching rate 85.2 ± 9.8%). There was a strong correlation between body weight (x) and time (y) needed from hormone injection to obtaining fertile oocytes (y = )0.8231x + 98.838 R 2 = 0.8428; P < 0.01). The cultured males ripened as early as at age 5 while females were 8-years old at first maturity.