Blast, caused by Magnaporthe grisea is one of most serious diseases of rice worldwide. A Chinese local rice variety, Digu, with durable blast resistance, is one of the important resources for rice breeding for resistance to blast (M. grisea) in China. The objectives of the current study were to assess the identity of the resistance genes in Digu and to determine the chromosomal location by molecular marker tagging. Two susceptible varieties to blast, Lijiangxintuanheigu (LTH) and Jiangnanxiangnuo (JNXN), a number of different varieties, each containing one blast resistance gene, Pik s , Pia, Pik, Pi-b, Pi-k p , Pi-ta 2 , Pi-ta, Pi-z, Pi-i, Pi-k m , Pi-z t , Pi-t and Pi-11, and the progeny populations from the crosses between Digu and each of these varieties were analysed with Chinese blast isolates. We found that the resistance of Digu to each of the two Chinese blast isolates, ZB13 and ZB15, were controlled by two single dominant genes, separately. The two genes are different from the known blast resistance genes and, therefore, designated as Pi-d(t)1 and Pid(t)2. By using bulked segregation method and molecular marker analysis in corresponding F 2 populations, Pi-d(t)1 was located on chromosome 2 with a distance of 1.2 and 10.6 cM to restriction fragment length polymorphism (RFLP) markers G1314A and G45, respectively. And Pi-d(t)2 was located on chromosome 6 with a distance of 3.2 and 3.4 cM to simple sequence repeat markers RM527 and RM3, respectively. We also developed a novel strategy of resistance gene analogue (RGA) assay with uneven polymerase chain reaction (PCR) to further tag the two genes and successfully identified two RGA markers, SPO01 and SPO03, which were co-segregated toPi-d(t)1 and Pid(t)2, respectively, in their corresponding F 2 populations. These results provide essential information for further utilization of the Digu's blast resistance genes in rice disease resistance breeding and positional cloning of these genes.U. S.

A new simple mechanism due to cold electron flow to produce strong magnetic field is proposed. A 600-T strong magnetic field is generated in the free space at the laser intensity of 5.7 × 10 15 ⋅ −2 . Theoretical analysis indicates that the magnetic field strength is proportional to laser intensity. Such a strong magnetic field offers a new experimental test bed to study laser-plasma physics, in particular, fast-ignition laser fusion research and laboratory astrophysics.

Based on the current deuterium metabolism imaging (DMI) technology analysis, a design scheme of a dual-core imaging coil suitable for a 7T MRI system is proposed. The size and structure of the shield layer are optimized. Based on the single-layer shield structure, a double-layer shield structure is proposed to improve the stability of the probe. A detuning circuit is added to the 2H transmitting and receiving coils so that they can work in time for better decoupling. According to the design scheme, the 1H/2H dual-core imaging coil was fabricated and applied to the 7T magnetic resonance system. We performed imaging experiments on D2O samples and quantitatively compared the correlation performance (SNR) of a 1H/2H dual-tuned RF coil with the same size deuterium transceiving-receiving integrated surface coil. The results verified that deuterium metabolism imaging using 1H/2H dual-tuned transmit-receiving separation MRI probe can effectively improve the signal-to-noise ratio of the target region.

Until now, the photoacoustic spectrum (PAS) analysis technology can't face the need for sulfur hexafluoride (SF6) gas-decomposition products due to the high noise level, big shock and low accuracy. In this manuscript, we present a portable SF6 gas-decomposition PAS gas analysis system based on mid-infrared quantum cascade laser (MI-QCL). Because the narrow linewidth, high speed tunable and stability wavelength of MI-QCL, our PAS gas analysis system has a good performance. A platform for MI-QCL PAS system is set up in our work. The accuracy of quantitative detection for sulfur dioxide (SO2), hydrogen sulfide (H2S) and carbon monoxide (CO) in SF6 gas background mixture gas is 0.5ppm, 0.1ppm and 0.1ppm, respectively. Experiment results demonstrate the MI-QCL PAS system not only has a high detection accuracy, but also has a small system volume. This work gives a novel solution method for PAS system miniaturization in the future.