Adenosine triphosphate pool during the growth cycle in Streptococcus faecalis. J. Bacteriol. 90:1013-1018. 1965.-The adenosine triphosphate (ATP) pool has been studied throughout the growth cycle of Streptococcus faecalis. Normally, the pool is quantitatively related to the concentration of organisms and the growth rate, but deviations from these relationships can occur without affecting the growth rate of the organisms. A critical concentration of ATP seems necessary for exponential growth, and, at lower levels, only linear growth can occur. If no growth can take place, catabolism of added energy source gives rise to a large increase in the pool level. The pool level represents the balance between the demands of the organisms for energy and the supply of energy derived from catabolism of the substrate.
, AND D. J. WALKER. Synthesis of reserve materials for endogenous metabolism in Streptococcusfaecalis. J. Bacteriol. 89:1448-1452. 1965.-The growth curve of Streptococcusfaecalis in batch culture with limited energy source shows an initial portion of exponential growth where the growth yield coefficient Yglucose is 32, followed by linear growth where ,Yglucose is 21. Endogenous metabolism is correlated with maintenance of glycolytic activity, and there is a marked change in the glycolytic activity of cells harvested under these conditions; however, endogenous metabolism cannot be detected in either case by measurement of the adenosine triphosphate (ATP) pool in the organisms. Cells harvested after growth with excess energy source exhibit endogenous metabolism, which is correlated with a much higher concentration of ATP in the organisms than occurs in the cells grown with limited energy source.
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