We monitored reptile and bird roadkills in Cerrado–Pantanal landscapes along the Campo Grande to Corumbá highway BR-262. We describe species distribution in different landscape zones, including the first geographic record for Hydrodynastes bicinctus Herrmann, 1804 in the Pantanal basin. The roadkill occurrence of Spizaetus melanoleucus (Vieillot, 1816) is an outstanding record. We recorded 930 individuals belonging to 29 reptile and 47 bird species; 20 of these species are new roadkill records in Brazil. The 8 new records of reptile species include Eunectes notaeus Cope, 1862, Bothrops mattogrossensis Amaral,1925, Dracaena paraguayensis Amaral,1950 and H. bicinctus; and 12 new records of bird species include S. melanoleucus, Heterospizias meridionalis Latham, 1790, Urubitinga urubitinga (Gmelin, 1788), Pulsatrix perspicillata (Latham, 1790), Aramus guarauna (Linnaeus, 1766), and Jabiru mycteria (Lichtenstein, 1819). Richness of road-killed species on the BR-262 highway seemed to be high, reinforcing concerns about wildlife-vehicle collisions where these accidents occur, as they lead to long term and chronic impacts on wildlife and road safety in the Pantanal region.

Great Kiskadees (Pitangus sulphuratus) are found in a variety of habitats from Argentina north to the United States and are the most generalist tyrant flycatcher in both foraging behavior and food habits. These kiskadees are known to occasionally prey on small vertebrates, but, to our knowledge, bats have never been reported as a prey item. We observed a breeding pair of Great Kiskadees preying on bats (Myotis spp.) at the field station Base de Estudos do Pantanal (BEP) in the southern Pantanal, Brazil. At BEP, there are fissures under the building's floor slabs that allow two species of bats, black myotis (Myotis nigricans) and silver-tipped myotis (M. albescens), to access internal galleries and use them as day roosts. We found that bats, insects, and fruits were the most common food items fed to nestlings by adult kiskadees. Bats (N = 10) were captured when kiskadees landed on ground below a building, looked up through a fissure, and then reached through the fissure and captured a bat in their bill. On one occasion, a kiskadee flew from a perch and captured a bat in flight. Our observations provide further evidence of the opportunistic feeding behavior of Great Kiskadees. RESUMEN. Depredación de murciélagos por Pitangus sulphuratusLos pitogués (Pitangus sulphuratus) se encuentran en una amplia variedad de hábitats desde Argentina hasta los Estados Unidos, y se le considera el más generalista de los tiránidos tanto en conducta de forrajeo como en su dieta. Se sabe que estas aves ocasionalmente depredan pequeños vertebrados, pero según nuestro conocimiento, nunca habían sido informados depredar murciélagos. Observamos un par de pitogués depredando murciélagos (Myotis spp.) en la estación de campo Base de Estudos do Pantanal (BEP) en el Pantanal sur, Brasil. En el BEP, hay fisuras bajo las planchas del suelo de los edificios que permiten que dos especies de murciélagos, Myotis nigricans y M. albescens, utilizen las galerías internas como lugares para pasar el día. Encontramos, que los murciélagos, insectos y frutas fueron los artículos alimentarios mas comúnmente utilizados para alimentar a los pichones. Los murciélagos (N = 1) fueron capturados cuando los pitogués aterrizaron en el suelo, miraron dentro de las fisuras y a través de estas alcanzaron a los murciélagos. En una de las ocasiones, una de las aves voló de una percha y capturo a un murciélago a vuelo. Nuestras observaciones proveen evidencia adicional sobre la conducta alimentaria oportunista del pitogué.