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This study investigated if cadmium chloride (CdCl 2 )-induced hepatic steatosis and fibrosis and the protective effect of quercetin (QUR) are mediated modulating the activity of miR-21, a known hepatic lipogenic and fibrotic miRNA. Male rats (n = 8/group) were divided as control, control + QUR (50 mg/kg; orally), CdCl 2 (10 moml/L; drinking water), CdCl 2 + miR-21 antagomir (inhibitor) (16 mg/kg/first 3 days), and CdCl 2 + QUR (50 mg/kg). Treatments were conducted for 20 weeks, daily. All treatments showed no effect on fasting glucose and insulin levels. Administration of either miR-21 or QUR prevented CdCl 2 -induced hepatic damage, as well as lipid droplets and collagen deposition. They also reduced serum levels of ALT and AST and decreased serum and hepatic levels of total cholesterol, triglycerides, and low-density lipoproteins in CdCl 2 -treated rats. Concomitantly, they reduced hepatic levels of reactive oxygen species, malondialdehyde, interleukin-6, and tumor necrosis factor-α, suppressed the activation of NF-kb P65, and increased hepatic levels of nuclear factor erythroid 2-related factor 2 (Nrf2), glutathione (GSH), and superoxide dismutase (SOD). These effects were associated with reduced expression of SREBP1, TGF-β1, Smad3, and collagen1 A and increased expression of PPARα, CPT1, and smad7. Interestingly, QUR significantly lowered levels of miR-21 and increased the protein levels and activity of Nrf2, as well as levels of GSH and SOD in the livers of both the control and CdCl 2 -treated rats. Of note, levels of Nrf2 were negatively correlated with the transcription of miR-21. In conclusion: QUR prevents CdCl 2 -induced hepatic steatosis and fibrosis mainly through attenuating its ability to upregulate miR-21, at least, by upregulation of Nrf2.

Entomopathogenic fungi are regarded as effective biocontrol agents in pest management. Different fungi isolates exhibit varying degree of pathogenicity against red palm weevil [Rhynchophorus ferrugineus (Olivier)]. The pathogenicity of four native isolate from Saudi Arabia (three Beauveria bassiana named as BbSA-1, BbSA-2, BbSA-3 and one Metarhizium anisopliae regarded as MaSA-1) and three exotic isolates from Indonesia (B. bassiana coded as BbIDN-1 and M. anisopliae named as MaIDN-1 and MaIDN-2) was evaluated against red palm weevil under laboratory conditions. The isolates were applied to eggs (1 day old), larvae (3 and 35 days old), pupae (5 days old) and adults (10 days old). The average mortality rate of eggs and hatched larvae was 100% in all of the isolates except BbSA-2 and BbIDN-1, where mortality was 93.3 and 90%, respectively. The lowest mortality rate (73.3%) was recorded for BbSA-3 against 3-days-old larvae; however, all other isolates caused >80% larval mortality. Meanwhile, 93.3% mortality of 35-day-old larvae was noted for MaSA-1 isolate. The highest pupa mortality (80%) was observed for MaSA-1, while remaining isolates caused >60% mortality. The isolates BbSA-1 and MaSA-1 caused 61 and 74.3% mortality in adults, respectively. The tested fungi isolates exhibited high virulence against all life stages of red palm weevil. Local isolates had higher pathogenicity than exotic isolates. The findings of the current study suggest that entomopathogenic fungi could be used as biological control agents for the management of red palm weevil. However, field studies are needed to reach the sound conclusions and practical applications.