Objective of the study was to evaluate the remineralizing effect of a prepared chicken eggshell powder (CESP) containing solution on human dentine and to compare it to that of light curing calcium hydroxide (Ca-OH) cavity liner. Chicken eggshell powder was prepared, characterized using a scanning electron microscope (SEM) and an energy-dispersive X-ray spectroscopy (EDX). A total of 24 patients were selected from operative dentistry clinic, each patient had at least two occlusal deep carious defects in permanent premolars or molars. After complete caries excavation, the 48 teeth were divided into two groups (n = 24): according to the received treatment: eggshell collagen delivering device (group 1) and light curing Ca-OH (group 2) as indirect direct pulp capping biomaterials. Teeth were then restored by a reinforced glass ionomer base and a permanent resin-based composite (RBC) filling. Cone beam computed tomography (CBCT) radiographs were used to evaluate and compare the variation in thickness of inducted dentine bridge immediately after insertion of final restoration and after 6months to evaluate thickness of dentine bridge finally the measurements were statistically evaluated by paired t test. Results showed that the group of teeth treated by pulp capping using the prepared remineralizing eggshell delivered by kolspon sponge showed a significantly higher thickness of dentin bridge formation than the group that was managed by application of conventional Ca-OH pulp capping agent. Concluding that A prepared chicken eggshell powder (CESP) in reminerization solution loaded into a collagen kolspon sponge might serve successfully in induction of newly formed reparative dentine bridge.