Goal: To describe the symptoms, diagnostic investigation and clinical management of a case of persistent neonatal hypoglycemia in Manaus, Amazonas. Methods: The proposed study is an observational and descriptive case report, developed in a private hospital in Manaus, started in October 2022. The instruments used for data collection were anamnesis and physical examination in the period in which the patient The patient remained hospitalized in the aforementioned hospital unit -both in the neonatal intensive care unit (neonatal ICU) and in the Clinical Nursing, through the review of the medical record. The observation and discussion of the case in the multidisciplinary meetings of the service were also part of the data acquisition. The completion of the information collection occurred through the acquisition of secondary data with continuous review of the electronic medical record and photographic record of the diagnostic methods to which the patient was submitted and bibliographic review. All the records and information obtained were duly explained and requested from the patient's mother, who by mutual agreement and without profit, signed an informed consent (ICF). Experience report: Reinforce, through a case report, the importance of clinical manifestations and complementary tests in the investigation of neonatal hypoglycemia in a child from the Western Amazon and describe the treatment established and the recommended clinical follow-up. Conclusion: Neonatal hypoglycemia can have a multifactorial origin and, if it is not properly identified and treated, it can have consequences on the child's neuropsychomotor development. Early diagnosis and proper management are essential, since they improve survival and avoid sequelae to the patient.
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