Here, a lipidated peptide Pal-pHK-pKV with self-assembly properties and the ability to provoke the disruption of the mitochondrial voltage-dependent anion channel-1 protein (VDAC1)-hexokinase-II (HK-II) complex is reported. The effects of the peptide pHK (N-terminal 15-amino acid fragment of HK-II that specifically binds VDAC1) are compared to those of a designed biomimetic amphiphilic pHK-pKV conjugate (pHK coupled with a cell-penetrating peptide pKV) and Pal-pHK-pKV (a lipidated conjugate modified with a hydrophobic palmitic (Pal) alkyl chain). The Pal-pHK-pKV exhibits a stronger interaction with the membrane as compared to pHK-pKV, which is demonstrated by the Langmuir–Blodgett technique and two-photon excitation microscopy. The amphiphilic peptide derivatives are cytotoxic to the A549 cells, but Pal-pHK-pKV is more cytotoxic. The inhibitory effects of the pHK derivatives on the A549 cells growth are investigated through induced apoptosis pathway, depolarized mitochondrial membrane potential, inhibited glycolysis, and activated caspase. The results of the immunofluorescence evidence the specific mitochondrial targeting by those derivatives.

Extensive fault information can be obtained from the vibration signals of rotating machines with faulty rolling bearings. However, the diagnosis of compound faults is challenging because of their easy mix-ups, which can lead to faulty diagnosis and judgment. This study improves the multichannel singular spectrum analysis (MSSA) by using convex optimization. In addition, an integrated fault diagnosis technology for rolling bearings using an improved MSSA and frequency–spatial domain decomposition (FSDD) was developed. This approach involves two primary stages: signal preprocessing and fault diagnosis. The proposed method was tested to diagnose faults in the rolling bearings of pellet mills. Signal preprocessing can significantly improve the quality of a vibration signal and preserve modal information that characterizes a fault. Fault diagnosis identifies the modal parameters entirely and accurately from the reconstructed vibration signal, and determines the degree of damage. The proposed method can aid in the robust diagnosis of faulty rolling bearings under severe operating conditions.

A test bench for integrative evaluation of the pneumatic activated ABS/TCS performance of a vehicle has been developed. The test bench is built based on the platform of Matlab/xPC. It can be used to test the performance of ABS/TCS ECU and the characteristic of pneumatic solenoid valve. It can also be used in guiding the design of a ABS/TCS product for original equipment manufactures (OEMs), or find the most suitable product among various market available ABS/TCSs for specific vehicle application for a vehicle manufacturer. The scheme of hardware and software of the bench is described in detail. The tests of braking and starting performance of a vehicle with ABS/TCS driving on the road with low adhesive coefficient and the road which experiences sharp changes in adhesive coefficient and the operating characteristic of the solenoid valve are taken as major test contents. The test results show that the bench can completely emulate the real operating characteristic of a ABS/TCS system as its operating on real roads

When braking on split-μ road, yaw moment and steering force of vehicle will be produced because the braking force is different for two side wheels. In order to deal with this problem, the modified independent control mode is widely applied, however, there also exits some defects. Because the gap of the adhensive coefficient of both sides is uncertain while braking, if the fixed modified coefficient is applied, it is difficult to keep vehicle direction stability and shorten the braking distance simultaneously under different gap former mentioned. This paper proposes a method named split-μ road identification. Compare the difference of slip integral of front wheels to threshold values during a certain pressure state in order to identify whether the vehicle brakes on split-μ road. According to the integral of slip from both side wheels and vehicle deceleration, the control mode dynamically changes the modified coefficients of pressure. Simulation results show that the algorithm can identify whether the vehicle is braking on split-μ road timely and also can identify the friction difference between the two sides accurately. The pressure regulation mode can regulate brake pressure appropriately and keep vehicle directional stability and shorten braking distance simultaneously.

No abstract