In this study, the biomechanical properties of human liver and spleen were studied by quantifying the damage mechanisms of various organs, the biomechanical response and the rate dependence of model materials. Used CIREN and NASS-CDS databases to determine the impact characteristics of liver and spleen injuries. The fresh human organs of ram type compression test and strain rate test were conducted to determine the optimum conditions for each organ of the finite element simulation. The results showed that the responses of human liver and spleen were nonlinear and rate dependent. This study will enhance the understanding of visceral organ damage, and provide a biomechanical basis for further establishing the finite element model of human internal organs.