This paper examines the recent introduction of the AIS to the ship's bridge and its potential impact on the safety of marine navigation. Research has shown that 80 to 85% of all recorded maritime accidents are directly due to human error or associated with human error. Safety is an important element of marine navigation and many people at different levels are involved in its management. The safe and efficient performance of joint systems, is heavily dependent upon how functions are allocated between the human and the machine. This paper investigates different regulations, supervision for proper use, training, and management of AIS users. It uses previous research and three separate AIS studies to identify problems. The potential of the AIS to cause problems is analysed. The classic human factor '' Swiss Cheese '' Model of system failure has been modified for the AIS to investigate a possible accident trajectory. The paper then concludes with recommendations and suggestions for improvements and further work. K E Y W O R D S 1. Human error. 2. AIS. 3. Marine navigation safety. 4. Training.

The RNA G-quadruplex is an important secondary structure formed by guanine-rich RNA sequences. However, its folding studies have mainly been studied in vitro. Accurate identification of RNA G-quadruplex formation within a sequence of interest remains difficult in cells. Herein, and based on the guanine-rich sequence in the 5'-UTR of NRAS mRNA, we designed and synthesized the first G-quadruplex-triggered fluorogenic hybridization (GTFH) probe, ISCH-nras1, for the unique visualization of the G-quadruplexes that form in this region. ISCH-nras1 is made up of two parts: The first is a fluorescent light-up moiety specific to G-quadruplex structures, and the second is a DNA molecule that can hybridize with a sequence that is adjacent to the guanine-rich sequence in the NRAS mRNA 5'-UTR. Further evaluation studies indicated that ISCH-nras1 could directly and precisely detect the targeted NRAS RNA G-quadruplex structures, both in vitro and in cells. Thus, this GTFH probe was a useful tool for directly investigating the folding of G-quadruplex structures within an RNA of interest and represents a new direction for the design of smart RNA G-quadruplex probes.

Children hospitalized for TBI have persistent dysfunction with unmet needs across multiple domains. After initial hospitalization, children with TBI should be monitored for functional impairments to improve identification and fulfillment of service needs.