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Background. Clinical studies have shown that ankylosing spondylitis (AS) could be significantly improved by Governor Vessel moxibustion (GVM) therapy. Objective. Study whether GVM therapy alleviates the clinical symptoms of AS by modulating intestinal microbiota. Methods. A total of 9 AS patients and 9 paired healthy individuals were enrolled, and GVM therapy was provided to the AS patients. Stool, urine, and saliva samples from the healthy individuals and the AS patients before and after GVM therapy were collected, and 16S rRNA gene sequencing was performed for microbiota analysis. Results. We found that GVM therapy can significantly alleviate the symptoms of AS, such as diarrhea, abdominal pain, and bloating. GVM therapy also decreased the abundances of Bacteroides and Prevotella while increasing the abundances of beneficial bacteria, such as Lactobacillus, in the gut microbiota of the AS patients. The analyses for AS clinical data and microbial abundances in AS patients revealed their multiple significant correlations ( P < 0.01 ); for example, an unclassified crystal was positively correlated with AF12 and Delftia, monocyte had a negative correlation with Scardovia, and human leukocyte antigen-B27 was negatively correlated with Catenibacterium, Coprococcus, and Oscillospira. Conclusions. Overall, these findings demonstrate that GVM therapy can alleviate AS clinical symptoms, and at the same time, it improves the microbial structure of microbiota in AS patients. This trial is registered with Chinese Clinical Trial Registry ChiCTR2100051907.

Objective: To investigate the musculoskeletal status and psychological status of female recruits during military boot enlistment training. Methods: The musculoskeletal status and psychological status of 110 boot training patients were assessed by scale, the psychological status of the subjects was assessed by Zung Anxiety Self-Rating Scale and Zung Depression Self-Evaluation Scale, the musculoskeletal status was assessed by visual analogue scale, Neck disability index, JOA low back pain evaluation form, Knee injury and Osteoarthritis Outcome Score scale, the medical document data during boot training were collated, and the risk factors affecting physical and mental health were analyzed. Thus, interventions are taken to reduce the injury rate and improve training performance.Results: Logistic regression analysis showed that neck dysfunction was a risk factor for depressive state and lumbar dysfunction; lumbar dysfunction was a risk factor for anxiety state, neck dysfunction and pain symptoms around the knee joint; pain around the knee joint was a risk factor for lumbar dysfunction and a protective factor for neck dysfunction; anxiety state was a risk factor for depressive state; depression state was a risk factor for anxiety state and neck dysfunction; and young age was a protective factor for anxiety state. Conclusion: In 10 weeks of military training, the site with the highest musculoskeletal injury is around the knee joint, followed by the neck and waist. Knee injury and Osteoarthritis Outcome Score scale scores were significantly associated with mental health status, with neck dysfunction increasing the risk of depression and lumbar dysfunction increasing the risk of anxiety in recruits. Prevention and treatment need to pay attention to these parts, gradually improve the balance, flexibility, strength of the body, while paying close attention to the mental health of recruits, so as to reduce the rate of injury. Keywords: recruits; military training; female soldiers; anxiety; depression; musculoskeletal injury.