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those of controls, p<0.05. The expression of IL-23 protein were also higher than those of controls, which in concordance with the changes of mRNA. Conclusions Our data show that local significantly increased levels of IL-23p19mRNA in myocardium and IL-23 may play a role in the pathogenesis of mice virus myocarditis. Objective To study the effect of the Mixture of Shengmai Pulvis and Danshen Decoction in protecting rats of the type 2 diabetic cardiomyopathy (DCM) model. Methods 42 SD rat models of DCM, established by combination of insulin resistance by a high-fat diet with intraperitoneal injection of high dose streptozotocin (50 mg/kg), were evaluated in the damage of the myocardium by ECG at the twelveth week after modelling, and the serum are analysed for blood glucose (GLU), cholesterol and triglyceride (TG); the content of the left cardiac ventricle myocardial collagen was quantified by Masson staining test; the level of myocardial cell apoptosis was tested with Tunel apoptosis kit; the damage extent of the myocardial subcellular structure was observed by electron microscopy; the expression levels of cardiac TSP-1, TGF-b1 and TRB-3 proteins were detected by immunohistochemistry, the changes of the expression levels of the cardiac TSP-1, A-TGFb1 and L-TGF-b1 protein were detected by Western blotting; and the changes of the mRNA expression levels of TSP-1 and TRB-3 were detected by real-time quantitative PCR. Results Compared with the control group, the rat blood glucose, cholesterol, triglycerides were significantly decreased; the myocardial tissue was less damaged and the collagen fibre content was reduced in the group of the Mixture of Shengmai Pulvis and Danshen Decoction; The myocardial sub-cellular structural damage in electron microscopy was to a lesser extent, the expression levels of the myocardial TSP-1, TGF-b1 and TRB-3 by immunohistochemical detection and the average expression levels of the myocardial TSP-1, A-TGFb1 and L-TGF-b1 by Western blotting were decreased; and the expression levels of TSP-1mRNA and TRB-3 mRNA by PCR detection were decreased than those of the control group. Conclusion The Mixture of Shengmai Pulvis and Danshen Decoction can inhibit through multiple pathways the process of myocardial fibrosis in the rat myocardium of diabetic cardiomyopathy, and significantly delay the formation course of diabetic cardiomyopathy in hyperglycemia rats. e0218 BIOLOGICAL CHARACTERISTICS RESEARCH OF STENT COATING WITH ZEDORAY CONSTITUENTS IN A PORCINE MODEL

To investigate the expression and distribution of catestatin and its precursor phromogranin A in tissue and cardiovascular cell levels. Method Extract RNA from Wistar rat heart tissue, vascular tissue, cardiomyocytes, cardiac fi broblasts, vascular smooth muscle cells and vascular adventitial fi broblasts by Trizol, detect of CST precursor chromogranin A mRNA levels by RT-PCR. CST protein level was detected by ELISA after homogenate and centrifugation. Results CST precursor chromogranin A mRNA expression and CST protein expression were detected in heart tissue, vascular tissue, cardiomyocytes, cardiac fi broblasts, vascular smooth muscle cells and vascular adventitial fi broblasts, CST protein expression was consistent with the mRNA expression of its precursor chromogranin A. Conclusion (1) Catestatin and its precursor chromogranin A could be expressed in heart and vascular in tissue and cell level, cardiovascular may be a new novel source for catestatin and its precursor chromograninA. (2) Catestatin from cardiovascular source could possibly inhibit catecholamine release from peripheral vascular neurons in a paracrine feedback manner to achieve its role for the regulation of cardiovascular function (negative inotropic effect and antihypertensive effect).