Nut fruits likely played a significant role before and during the origin of agriculture; however, relatively little research conducted on the morphological characteristics and statistical comparisons of nut fruit starch granule hinders the progress of paleodietary analysis of prehistorical society. For better species identification of starch granule remaining on tools discovered at archaeological sites, it is desirable to develop a more abundant morphology database of modern nut fruit starch granules as well as the establishment of relevant identification standards. Therefore, nuts from 40 species in four genera (Quercus, Lithocarpus, Castanea, and Castanopsis) of Fagaceae were collected from South China for statistical measurement and comparative analysis. Starch granules are highly accumulated in 34 species except for 6 species, whose shapes involve oval, subcircular, drop-shaped, rounded triangle, polygonal, spherical caps, and bell-shaped types, or a combination of several types, and the average length is between 10 and 20 μm. According to research on Quercus phylogeny relationships, it was found that the species in the same infragenious section produce similar morphological characteristics of starch granules. The result was applied in the identification of starch granules extracted from stone tools from the 20 to 10 ka cultural layer of Xiaodong Rockshelter, and some starch granules can be recognized to species level, revealing that nuts from Quercus and Lithocarpus were gathered and exploited by ancient people. This expansion of modern starch presentation and comparison of nuts helps to improve the accuracy of the identification of ancient starch and deepen the understanding of plant utilization of ancient humans.

Phytoliths, as a newly developing plant proxy, have broad application prospects in paleoclimate and paleoethnobotany. However, the shortage of studies regarding tropical-subtropical plants and topsoil phytoliths interferes with the research progress on primitive humanity’s utilization of plant resources and paleoclimate in the region. This research focuses on the subtropical mountainous region with a monsoon climate of low latitudes in Southwest China to conduct phytolith morphology analysis of living plants and phytolith/pollen assemblages of topsoil to reveal the indicative significance of vegetation and climate. A total of 111 species from 50 families, including 73 species from 33 tree/shrub families, 31 species from 12 herb families and 7 species from 5 fern families, were collected for morphological characteristics analysis, as well as 19 topsoil specimens for phytolith and pollen assemblage analysis. The results suggest that phytoliths are mainly deposited in situ, with assemblages of topsoil corresponding well with plant types in the quadrat and being able to exhibit constructive species in small regions. In comparison, pollen assemblages of topsoil dominantly respond to regional vegetation due to their long-distance transportation and widespread presence, in addition to their characteristics that correspond to the vegetation in the quadrat. The topsoil phytolith assemblages are mainly based on the elongate-bulliform flabellate-square/rectangle-broadleaf-types (including spheroid echinate), and the vegetation types indicate the subtropical climate. In addition, phytolith assemblages of Poaceae are dominated by collapsed saddle-bulliform flabellate square/rectangle-elongate-point, reflecting warm and humid conditions. The pollen assemblages mainly consist of Pinus, Betula, Alnus, deciduous Quercus, Euphorbiaceae, Rhamnaceae and Polygonum, reflecting tropical-subtropical plant communities and indicating warm and humid conditions. Overall, phytolith and pollen assemblages have unique characteristics and are thus explicitly representative of the low-latitude subtropical monsoon climate.